B-cells ensure humoral immune response against antigens (Ag) thanks to their receptor (BCR). V(D)J rearrangement, somatic hypermutation, immunoglobulin (Ig) class switch and locus suicide recombination are mutational/recombinational processes targeting Ig loci influencing BCR expression. Study of these events is essential for B cell function analysis. Our project will provide the normal reference values using high throughput sequencing-based protocols.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
OTHER
Masking
NONE
Enrollment
120
The blood samples will be collected from healthy volunteers : 7 tubes of 7 ml with anti-coagulant Héparine Lithium and 1 tube of 7ml with anti-coagulant EDTA
Limoges University Hospital
Limoges, France
Frequency of use of the V, D and J genes in VDJ rearrangements
Time frame: through study completion, an average of 18 months
Percentage of HyperMutation Somatic (SHM) in VDJ regions
Time frame: through study completion, an average of 18 months
Nature of HyperMutation Somatic (SHM)
transitions and transversions
Time frame: through study completion, an average of 18 months
Ig class switching (CSR) and recombination suicide of the IgH locus (LSR) junctions
number of CSR and LSR junctions
Time frame: through study completion, an average of 18 months
Frequency of g class switching (CSR) and recombination suicide of the IgH locus (LSR) junctions
frequency of CSR and LSR junctions according to their structure
Time frame: through study completion, an average of 18 months
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.