This study is to find out the association between gut microbiota and moyamoya disease.
Moyamoya disease (MMD) is a rare cerebrovascular disorder, it is a major cause of stroke in children and young adults in Japan, Korea, and China, but its pathogenesis is not yet clear. Dysbiosis of gut microbiota may play a role in the pathological change of MMD. In this research, the investigators would perform metagenomic sequencing to find the characteristics of gut microbiota of MMD and to explore the correlations with metabolic, immune, and clinical symptoms. This research will not only help the understanding of the pathophysiology of MMD, but also may provide a reference for the selection of clinical treatment options.
Study Type
OBSERVATIONAL
Enrollment
120
Beijing Tiantan Hospital Capital Medical University
Beijing, Beijing Municipality, China
RECRUITINGDiversity analysis of genes and species between MMD patients and the control group
Based on the gene and species composition of each sample, the Chao1 and Shannon indexes, as well as the observed OTUs (operational taxonomic units), will be calculated in order to identify the differences in gene and species diversity for each group.
Time frame: Through study completion，eight months.
Analysis of differences in intestinal microbiota between MMD patients and the control group
The Spearman correlation coefficient between genes will be calculated, and genes with strong correlation will be grouped into one cluster, as a CAG. The abundance of CAGs in each sample will be determined Furthermore, the significantly enriched species in the control and MMD groups will be enumerated for network display.
Time frame: Through study completion，eight months.
Analysis of functional differences in the intestinal microbiota of MMD patients in comparison to the control group
The LEfSe discriminant analysis will be used to screen for significant differences between groups. The dimensionality reduction will be implemented by LDA, and the impact of function difference will be evaluated to obtain the LDA score and identify significantly different functions between groups.
Time frame: Through study completion，eight months.
Analysis of metabolites of the intestinal microbiota of MMD patients in comparison to the control group
Metabolites of the intestinal microbiota between two groups
Time frame: Through study completion，eight months.
Correlation analysis between gut microbiota in MMD patients and inflammatory cytokines.
IL-4,IL-10,IL-13, IFN-α, TGF-β, IL-1,IL-6, IL-17, IFN-β, IFN-γ and TNF-α
Time frame: Through study completion，eight months.
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Correlation analysis between gut microbiota in MMD patients and neuroimaging markers and cognitive status.
MRI: BOLD, DWI, QSM, CUBE, ASL, T1, T2; Cognitive status: Wisdom, Attention, Memory, Linguistic fluency;
Time frame: Through study completion，eight months.
The differences of composition of gut microbiota among the genotypes (RNF213 p.R4810K)
RNF213 p.R4810K
Time frame: Through study completion，eight months.