Dietary protein is vital for the preservation of health and optimal adaptation to training. Plant proteins are considered inferior to animal proteins with respect to their ability to stimulate an acute muscle building response and therefore support long-term muscle reconditioning. Pea protein is a highly commercially available plant proteins source (available as supplements, food ingredients etc.), yet there is no research investigating its ability to stimulate a muscle building response. The investigators aim to assess the effect of consuming pea protein on muscle protein synthesis rates and compare these results to mycoprotein, a source known to elicit a robust anabolic response. Pea protein is lower in some of the essential amino acids, namely methionine, which could mean it is less effective compared with mycoprotein which has a more complete amino acid profile. So in addition to comparing pea with mycoprotein, the investigators also want to compare to a blend of pea and mycoprotein to see if replenishing the amino acid content in pea 'rescues' the anabolic response.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
TRIPLE
Enrollment
33
Ingestion of 25g of protein
University of Exeter
Exeter, United Kingdom
Muscle protein synthesis
The rate of synthesis of new muscle protein (Fractional synthetic rate %/h)
Time frame: 8.5 hours
Plasma amino acid kinetics
The appearance of amino acids from the protein drink in the circulation.
Time frame: 8.5 hours
Serum insulin concentrations
Basal and postprandial concentrations of serum insulin
Time frame: 8.5 hours
Translocation of mTOR via immunohistochemistry
Locality of mTOR within the muscle cell
Time frame: 8.5 hours
mTOR phosphorylation via ATP kinase assay
The amount of mTOR that has been activated in the muscle cell
Time frame: 8.5 hours
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