Endothelium Dependent and Non-dependent Vasodilatation in Human Gingiva

Semmelweis University24 enrolled

Overview

The aim of this study is to compare the endothelim-dependent and non-dependent vasodilatation between genders in the human gingiva.

Gender differences were observed in studies according to gingival microcirculation between physiological circumstances and during wound healing as well. For blood flow measurement Laser Spackle Contrast Imaging is a reliable tool. It has good reproducibility, it doesn't get in touch with the measured surface, it's easy to use and it can measure on a bigger surface in same time. Two wells were fabricated on the labial surface of the right lateral incisor (test tooth) and on the labial surface of the left central incisor (control tooth). These wells are opened towards the high-prermeability gingival sulcus, so the dropped solutions nitroglycerine vs physiological saline or nitroglycerine vs physiological saline can esasily penetrate to the gingival tissues. After applying the solutions, the blood flow was monitored fot 15 minutes.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

SINGLE

Enrollment

Conditions

Vasodilatation Oral Soft Tissue Conditions

Interventions

NitroglycerinDRUG

Vasodilatator solution is dropped on the labial surface on the lateral incisor, so it can penetrate through the sulcus.

AcetylcholineDRUG

Vasodilatator solution is dropped on the labial surface on the lateral incisor, so it can penetrate through the sulcus.

Eligibility

Sex: ALLMin age: 20 YearsMax age: 30 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * general health * minimum 5 mm keratinized gingiva at the upper front teeth * good oral hygiene Exclusion Criteria: * pregnancy and breast-feeding * smoking * medication * insufficient marginal integrity * periodontitis * caries

Locations (1)

Semmelweis University, Department of Conservative Dentistry

Budapest, Hungary

Outcomes

Primary Outcomes

Blood flow change after applying the test solutions

The change in blood flow will be measeured after applying the test solutions.

Time frame: 20 minutes

Secondary Outcomes

Comparison the change in blood flow between the two solutions

The blood flow values will be evaluated after each applied solutions.

Time frame: 30 minutes

Comparison the change in blood flow in genders

The maximal blood flow changes assessed in primary outcome will be split into two groups based on gender. The change will be compared between males and females.

Time frame: 20 minutes

Data from ClinicalTrials.gov

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