Purpose: To compare the effects of the density gradient centrifugation and microfluidic chip sperm preparation methods on embryo development in a study population with astheno-teratozoospermia. Materials and Methods: In this prospective study, the semen samples of the patients with astheno-teratozoospermia were divided into two groups for preparation with either the microfluidic or density gradient methods. Selected spermatozoa were used to fertilize the mature sibling oocytes. Semen parameters and embryo development on days 3 and 5 were assessed.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
SINGLE
Enrollment
22
Fertile Ultimate® (Koek Biotechnology, Turkey) microfluidic sperm sorting chips
Density gradient centrifugation
MaltepeU
Istanbul, Turkey (Türkiye)
Semen parameters after sperm preparation with either method: semen volume
semen volume (ml)
Time frame: up to 7 months
Semen parameters after sperm preparation with either method: sperm concentration
sperm concentration (106/ml)
Time frame: up to 7 months
Semen parameters after sperm preparation with either method: total motility
total motility (%)
Time frame: up to 7 months
Semen parameters after sperm preparation with either method: progressive motility
progressive motility (%)
Time frame: up to 7 months
Embryo development after sperm preparation with either method
Embryo development and blastocyst formation were evaluated on days 1, 3 and 5 based on the criteria reported by Veeck and Zaninovic, 2003
Time frame: up to 7 months
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