Cartilage Injury and Remodeling After ACL Rupture and Reconstruction: Functional Imaging and Biomarkers

Peking University Third Hospital76 enrolled

Overview

The proposed study will establish novel relationships between intra-articular MSC recruitment, synovial inflammation, biomarkers of cartilage degeneration and joint inflammation, clinical patient factors, and downstream alterations in cartilage composition and morphology to provide novel insights into the pathoetiology of PTOA after ACL injury and reconstruction.

In efforts to study the pathoetiology of post-traumatic osteoarthritis (PTOA), we propose a collaborative, prospective cohort study of 38 patients undergoing primary anterior cruciate ligament (ACL) reconstruction after ACL rupture. We will assess the longitudinal progression of patient-reported outcomes, knee laxity, MRI-based articular cartilage morphology and composition, and serum-borne biomarkers of cartilage degeneration up to 1 year of follow-up. Furthermore, stem cell mobilization, chemokine and pro-inflammatory cytokine concentrations, and inflammatory cell recruitment into synovial tissue will be measured from intraoperatively-collected samples. We will perform mixed multivariate linear regression modeling to elucidate potentially novel relationships between patient demographics, anatomy, biological factors, and downstream alterations in articular cartilage morphology and composition. We request $200,000 in funding over a two-year duration.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Anterior Cruciate Ligament Injuries

Interventions

ACL reconstructionPROCEDURE

All patients will undergo a standardized single-bundle technique with a hamstring or bone-patellar tendon-bone autograft.

Eligibility

Sex: ALLMin age: 18 YearsMax age: 30 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * \- Males and females, aged 18 - 30 years * Isolated, traumatic primary anterior cruciate ligament rupture requiring surgical reconstruction * Surgical reconstruction performed using a single-bundle technique utilizing an autograft * No history of ipsilateral traumatic knee injury or fracture * No evidence of PCL injury or more than grade 1 injury to the MCL or LCL Exclusion Criteria: * \- BMI \< 18.5 or \>35 kg/m2 * Injury occurred longer than 4 weeks before enrollment * Intra-articular steroid injection within 3 months of injury * Chondral defects greater than 3 cm2 or Outerbridge grade II or higher lesions

Locations (1)

Peking university third hospital

Beijing, Beijing Municipality, China

RECRUITING

Outcomes

Primary Outcomes

Objectives measure of knee stability

Knee stability (anterior-posterior laxity) will be measured utilizing the KT-2000 arthrometer, based on the side-to-side difference (SSD) observed between the injured and the healthy, contralateral knee. Subjects will be tested at 90 days, 180 days, and 1 year after surgery, with maximum manual force performed at 30 and 90 degrees of knee flexion.

Time frame: 3 months, 6 months, 12 months

COMP

Cartilage Biomarker Analysis;