Vaginal Microbiome-host Interaction in Mesh Exposure After TVM Surgery

Inclusion Criteria: * Patients with "III-IV degree uterine/apex/anterior vaginal wall prolapse and I-II degree of posterior vaginal wall prolapse" who agree to be treated by transvaginal pelvic mesh implantation. * All surgeries were performed by surgeons with extensive experience in transvaginal mesh implantation (\>20 vaginal mesh implantation per year) using macroporous, monofilament, polypropylene mesh. Exclusion Criteria: * 1\. Have had sex in the past 5 days or had vaginal irrigation; * 2\. Use oral or vaginal antibiotics, antifungal, immunosuppressant, hormone drugs or probiotics for the last 30 days. * 3\. Combined with immunosuppressive diseases, diseases with contraindication of vaginal estrogen such as breast cancer, endometrial cancer. * 4\. If the patient has a uterus before surgery and does not undergo total transvaginal hysterectomy during surgery, * 5\. Intraoperative bladder injury or intraoperative hemorrhage \>500ml or postoperative hematoma.