In Vitro Drug Sensitivity Testing of Fresh Human Samples

Hospices Civils de Lyon800 enrolled

Overview

Hematological malignancies gather several various pathologies included myeloproliferative disorders (as acute myeloid leukemia (AML) or chronic myeloid leukemia (CML)) and lymphoproliferative disorders (as chronic lymphoid leukemia (CLL), non-Hodgkin lymphoma (NHL) and multiple myeloma (MM)) . Over the last decade, the treatments have evolved significantly but the overall survival remains limited, especially for the AML and MM patients. There's an ongoing imperative to continue in-vitro and in-vivo studies to better evaluate the anti-cancer drugs sensitivity and therefore improving the response to treatments and open new fields of application. The healthy control group will be contributing to produce some pertinent and significant data for the results of the index cases group. The investigators aim to analyze 10 differents drugs, common use in chemotherapy or immunotherapy and evaluate by 2 ways the sensitivity: first, Flow cytometry (cells apoptosis by Annexine V and propidium iodide) on fresh samples and in a second time, monitoring of the blasts in the mouse's blood after injection.

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

OTHER

Masking

NONE

Enrollment

800

Conditions

Myeloproliferative Disorders Lymphoproliferative Disorders

Interventions

Blood collection for the evaluation of the anti-drugs sensitivityBIOLOGICAL

The M.D investigators and the medical team identify the patients ( index cases and healthy volunteers) in a medical appointment. They inform them, collect the non-opposition right and the blood samples: 5 mL heparin tube and 5 mL dry tube for each person , one time.

Eligibility

Sex: ALLMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Index cases: patients with myeloproliferative or lymphoproliferative disorders. * Controls: patients without hematological disease, without chemotherapy ((≤ 5 years.) and without immunosuppressive treatment at the study time. * Age ≥ 18 y.o for all the patients Exclusion Criteria: * Age \< 18 y.o * Pregnant or breastfeeding woman * Patients deprived of their liberty by judicial or administrative decision.

Outcomes

Primary Outcomes

Evaluation of the sensitivity of 10 anti-cancer drugs

Sensitivity determined by in-vitro * Tumor cells mortality by Flow cytometry * Monitoring of the blasts in the mouse's blood after injection

Time frame: Through study completion, an average of 3 years