The primary aim of the current study is to determine the effect of beetroot juice consumption on plasma trimethylamine N-oxide concentration. A secondary aims: exploring the effect of circulating trimethylamine N-oxide modification on plasma total antioxidant status, and measurement of changes in the skin nicotinamide-adenine dinucleotide reduced form (NADH) content during transient ischemia and reperfusion.
In a random order subjects will consume beetroot juice (140ml), or equimolar nitrates (NaNO3). Antecubital vein blood samples will be taken at the beginning, and every hour for 4 hours following consumption. Plasma trimethylamine N-oxide, total antioxidant status, and nitrite/nitrate concentrations will be determined. Using Flow Mediated Skin Fluorescence skin NADH content determined during controlled ischemia triggered by total occlusion of the brachial artery by the brachial blood pressure cuff and during reperfusion after deflation of the blood pressure cuff 2 and 4 hours following consumption.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
NONE
Enrollment
20
single intake of beetroot juice containing 800mg nitrates
single intake of 800mg nitrates
Akademia Wychowania Fizycznego
Poznan, Poland
The effect of beetroot juice consumption on plasma trimethylamine N-oxide concentration
Determination of plasma trimethylamine N-oxide concentration following the beetroot juice consumption using ultra performance liquid chromatography - tandem mass spectrometer (UPLC-MS/MS) method.
Time frame: 4 hours
The effect of circulating trimethylamine N-oxide modification on plasma total antioxidant status
Determination of plasma total antioxidant status following the beetroot juice consumption using commercially available kit.
Time frame: 4 hours
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