Obesity manifest with inflammation, hyperglycaemia and dyslipidaemia. These conditions disturb redox system by generating excessive reactive oxygen species (ROS) and causing oxidative stress (OS) leading to DNA damage. Very low calorie diet (VLCD) have rapid positive effect on weight loss, glucose homeostasis, inflammation and OS. The aim of study is to test the influence of 3-weeks VLCD on anthropometric, biochemical and genomic parameters in class II and III obesity patients.
Obesity is a chronic disease associated with chronic inflammation, insulin resistance, dyslipidemia, oxidative stress and increased risk for type 2 diabetes, cardiovascular disease, stroke and multiple cancer types. Oxidative stress can lead to base lesions of DNA which could be detected with alkine comet assay and its version with use of formamidopyrimidine DNA glycosylase-endonuclease enzyme in fresh or frozen small volume samples. Dietary caloric restriction has beneficial effects on insulin sensitivity, inflammation, oxidative stress and DNA repair. Very low calorie diet (VLCD) data are scarce, especially on the changing levels of DNA damage.This study will assess the effect of a three-week VLCD used in Special Hospital for extended treatment of Duga Resa in individuals with BMI ≥ 35kg/m2 on the level of primary and oxidative DNA damage as well as body composition and biochemical parameters.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
TREATMENT
Masking
NONE
Enrollment
22
During 3 weeks in hospital patients will eat prepared very low calorie diet composed of 50-60% complex carbohydrates with low carbo-glycemic index, 20-25% proteins and 25-30% fat
Special Hospital for Extended Treatment of Duga Resa
Duga Resa, City of Zagreb, Croatia
The changes in the body mass index
Body mass index (kg/m2) is calculated by using measured body weight (kg) with measured body height (m)
Time frame: Baseline, after 3 weeks of VLCD
The changes in the body fat mass
Body fat mass (kg) assessed with bioelectrical impedance method
Time frame: Baseline, after 3 weeks of VLCD
The changes in the skeletal muscle mass
Skeletal muscle mass (kg) assessed with bioelectrical impedance method
Time frame: Baseline, after 3 weeks of VLCD
The changes in the percent body fat
Percent body fat (%) assessed with bioelectrical impedance method
Time frame: Baseline, after 3 weeks of VLCD
The changes in fasting glucose concentration
Concentration of glucose (mmol/L)
Time frame: Baseline, after 3 weeks of VLCD
The changes in urea concentration
Concentration of urea (mmol/L)
Time frame: Baseline, after 3 weeks of VLCD
The changes in insulin concentration
Concentration of insulin (mIU/L)
Time frame: Baseline, after 3 weeks of VLCD
The changes in HOMA index
HOMA index is calculated according to the formula: glucose (mmol/L) x insulin (mIU/L)/22.5
Time frame: Baseline, after 3 weeks of VLCD
The changes in lipid profile
Concentrations of triglycerides (mmo/L), LDL (mmol/L), HDL (mmol/L) cholesterol (mmol/L)
Time frame: Baseline, after 3 weeks of VLCD
The changes in inflammation parameters
Concentration of C-reactive protein (mg/L)
Time frame: Baseline, after 3 weeks of VLCD
The changes in inflammation parameters
Concentration of total white blood cell count
Time frame: Baseline, after 3 weeks of VLCD
The changes in DNA damage assessed with alkaline comet assay
Values for alkaline comet assay in μm for tale length
Time frame: Baseline, after 3 weeks of VLCD
The changes in DNA damage assessed with alkaline comet assay
Values for alkaline comet assay in % for tale intensity
Time frame: Baseline, after 3 weeks of VLCD
The changes in oxidative DNA damage assessed with FPG comet assay
Values for FPG comet assay in % for tale intensity
Time frame: Baseline, after 3 weeks of VLCD
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