The purpose of this study was to determine the effectiveness of a combination of intranasal conditioned medium (CM) with intraparenchymal umbilical cord mesenchymal stem cells (UC-MSCs) transplantation in acute stroke patients to induce neurogenesis.
This study has 3 arms namely CM and UC-MSCs treatment, UC-MSCs treatment only, and control. The investigator hypothesized that group of CM and UC-MSCs combination is the optimal treatment to induce neurogenesis in stroke patients.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
NONE
Enrollment
15
Intranasal of 3 cc of conditioned medium each, for 3 days in a row
Intra-parenchymal transplantation of 20x10\^6 UC-MSCs
Such as clopidogrel, piracetam, citicoline
Gatot Soebroto Hospital
Jakarta Pusat, DKI Jakarta, Indonesia
RECRUITINGPT Prodia StemCell Indonesia
Jakarta, Indonesia
RECRUITINGChange from baseline Brain-Derived Neutrophic Factor at one month, 3 months and 6 months after transplantation
Venous blood collection
Time frame: Before treatment, one month after treatment, 3 months after treatment, and 6 month after treatment
Change from baseline Vascular endothelial growth factor at one month, 3 months and 6 months after transplantation
Venous blood collection
Time frame: Before treatment, one month after treatment, 3 months after treatment, and 6 month after treatment
Change baseline Magnetic Resonance Imaging at 6 month after transplantation
To observe the brain development (neurogenesis)
Time frame: Before treatment, 6 month after transplantation
The National Institute of Health Stroke Scale (NIHSS) Scoring
The NIH Stroke Scale (NIHSS) is a 15-item scale that is a well-validated and prognostically important measure of stroke-related neurologic deficits in research and clinical care
Time frame: Before treatment, one month after treatment, 3 months after treatment, and 6 month after treatment
modified Rankin Scale (mRS)
The Modified Rankin Scale (mRS) is used to measure the degree of disability in patients who have had a stroke
Time frame: Before treatment, one month after treatment, 3 months after treatment, and 6 month after treatment
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