Skin Responses and T Cell Immunology After House Dust Mite Exposure in Sensitized Atopic Dermatitis Patients

Inclusion Criteria: 1. Able and willing to give written informed consent. 2. Male and female subjects, aged 18-65 years. Women will be considered for inclusion if they are: 3. Not pregnant, as confirmed by pregnancy test (see flow chart), and not nursing. 4. Of non-child bearing potential (i.e. physiologically incapable of becoming pregnant, including any female who is pre-menarchial or post-menopausal, with documented proof of hysterectomy or tubal ligation, or meets clinical criteria for menopause and has been amenorrhoeic for more than 1 year prior to the screening visit). 5. Of childbearing potential and using a highly effective method of contraception during the entire study (vasectomised partner, sexual abstinence - the lifestyle of the female should be such that there is complete abstinence from intercourse from two weeks prior to the start of the study until at least 72 hours after the last study visit -, implants, injectables, combined oral contraceptives, hormonal IUDs or double-barrier methods, i.e. any double combination of IUD, condom with spermicidal gel, diaphragm, sponge, and cervical cap). 6. Positive IgE level for HDM of at least CAP FEIA class 3 (≥3,50 kU/l) at screening or in previous year. 7. Atopic dermatitis fulfilling the UK criteria of AD 8. SCORAD index between 20 and 50 points. 9. Positive self-history regarding poor skin condition during fall and winter months and allergic rhinitis and/or conjunctivitis in situations of significant HDM exposure (e.g., dusting). 10. FEV1 ≥ 80% pred. at screening. 11. Total Nasal Symptom Score (TNSS) of ≤ 3 prior to entering the chamber at visit 2. 12. Smokers or non-smokers. 13. BMI ≥18 and ≤ 35. Exclusion Criteria: 1. Any clinically relevant abnormal findings in physical examination, clinical chemistry, hematology, vital signs or lung function at screening visit , which, in the opinion of the investigator, may either put the subject at risk because of participation in the study or may influence the results of the study, or the subject's ability to participate in the study 2. Past or present disease, which as judged by the investigator, may affect the outcome of this study. These diseases include, but are not limited to, cardiovascular disease, malignancy, hepatic disease, renal disease, hematological disease, neurological disease, endocrine disease or pulmonary disease. 3. Asthma other than mild asthma which is treated with short acting beta-2-agonists only and which is controlled according to the current GINA guidelines 4. Subject with concomitant allergies to seasonal aeroallergens which become active (i.e., grass, trees, weeds, rye; defined as being symptomatic to aeroallergens within the past 2 years or within the past 2 allergy seasons) during the individual study participation. 5. Positive HIV-1/2Ab, hepatitis B surface antigen (HBsAg) or hepatitis C virus antibodies (HCV-Ab) test at screening or test not performed. 6. Treatment with medication that might interfere with rescue medication for anaphylactic reactions (e.g. beta blocker). 7. Topical steroid treatment (wash out phase: 2 weeks before day 1) 8. Topical calcineurin inhibitor treatment (wash out phase 2 weeks before day 1) 9. UV radiation treatment (wash out phase 4 weeks before day 1) 10. Systemic immunosuppression treatment (steroids, biologics, e.g. dupilumab, JAK-Inhibitors, cyclosporine, azathioprine, Mycophenolat Mofetil (MMF); wash out phase 4 weeks and 3 months, respectively, before day 1. 11. Treatment with antihistamines (wash out phase 1 week). 12. Unstable AD during Screening (SCORAD difference of \>10 points from Visit 1 to Visit 2) 13. Diastolic blood pressure above 95 mmHg. 14. Acute respiratory infection 2 weeks prior to screening visit. 15. Alcohol or drug abuse within 12 month prior to screening. 16. Regular daily consumption of more than 1 liter of usual beer or the equivalent quantity of approximately 40 g of alcohol in another form. 17. Participation in another clinical trial 30 days prior to enrollment. 18. There is a risk of non-compliance with study procedures. 19. Suspected inability to understand the protocol requirements, instructions and study-related restrictions, the nature, scope, and possible consequences of the study. 20. History of an acute infection four weeks prior to the informed consent visit. 21. Subject has received previous immunotherapy treatment with any HDM allergen within 3 years prior to screening. 22. Subject is receiving ongoing treatment with any specific immunotherapy for other allergies.