Obesity leads to physiological imbalance resulting in hyperglycemia, dyslipidaemia and inflammation and can generate systematic oxidative stress through multiple biochemical mechanisms. Oxidative stress (OS) can induce DNA damage and inhibit DNA repair mechanisms. Very low calorie diet (VLCD) have rapid positive effect on weight loss, glucose homeostasis, insulin resistance, inflammation and OS. The aim of this study is to determine the effect of a three-week VLCD on anthropometric, biochemical and genomic parameters in individuals with BMI ≥ 35kg/m2.
Obesity is a complex chronic multifactorial disease associated with concomitant or increased risk for chronic inflammation, insulin resistance, dyslipidemia, oxidative stress, type 2 diabetes, cardiovascular disease, stroke and multiple cancer types. Oxidative stress (OS) can cause permanent DNA damage which could be detected with lymphocytes cytokinesis-block micronucleus (L-CBMN) cytome assay. Weight loss and improvement of dietary habits in people with obesity can affect genome stability and have beneficial effects on insulin sensitivity, inflammation and OS. Effects of very low calorie diet (VLCD) on DNA damage are scarce. The aim of this study is to determine the effect of a three-week VLCD used in Special Hospital for extended treatment of Duga Resa in patients with BMI ≥ 35kg/m2 on permanent DNA damage, lipid profile, insulin resistance, inflammation and anthropometric parameters.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
TREATMENT
Masking
NONE
Enrollment
26
In hospital patients will eat prepared diet with 567 kcal a day during 3 weeks
Special Hospital for Extended Treatment of Duga Resa
Duga Resa, Croatia
The changes in body mass index
Body mass index is calculated by dividing body mass (kg) with square of body height (m)
Time frame: Baseline, after 3 weeks of VLCD
The changes in the body fat mass
Body fat mass (kg) assessed with bioelectrical impedance method
Time frame: Baseline, after 3 weeks of VLCD
The changes in the skeletal muscle mass
Skeletal muscle mass (kg) assessed with bioelectrical impedance method
Time frame: Baseline, after 3 weeks of VLCD
The changes in the percent body fat
Percent body fat (%) assessed with bioelectrical impedance method
Time frame: Baseline, after 3 weeks of VLCD
The changes in fasting glucose concentration
Concentration of glucose (mmol/L)
Time frame: Baseline, after 3 weeks of VLCD
The changes in urea concentration
Concentration of urea (mmol/L)
Time frame: Baseline, after 3 weeks of VLCD
The changes in lipid profile
Concentrations of triglycerides (mmo/L), LDL (mmol/L), HDL (mmol/L) cholesterol (mmol/L)
Time frame: Baseline, after 3 weeks of VLCD
The changes in insulin concentration
Concentration of insulin (mIU/L)
Time frame: Baseline, after 3 weeks of VLCD
The changes in homeostatic model assessment (HOMA) index
HOMA index is calculated according to the following formula: glucose (mmol/L) x insulin (mIU/L)/22.5
Time frame: Baseline, after 3 weeks of VLCD
The changes in inflammation parameters
Concentration of C-reactive protein (mg/L) and total white blood cell count
Time frame: Baseline, after 3 weeks of VLCD
The changes in oxidative stress
Concentration of glutathione (RFU) and reactive oxygen species (RFU)
Time frame: Baseline, after 3 weeks of VLCD
The changes in DNA damage
Frequency of micronucleus, nuclear buds, nucleoplasmic bridges, apoptotic and necrotic cells among 1000 lymphocytes
Time frame: Baseline, after 3 weeks of VLCD
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