Thyroid Gland Dysfunction and Vitamin D Polymorphism in Keratoconus

Mansoura University262 enrolled

Overview

This prospective study the thyroid gland dysfunction and vitamin D receptor polymorphism in keratoconus patients

This is a prospective observational study in which blood samples are collected from keratoconus patients looking for thyroid dysfunction and vitamin D levels in the serum and assessing the Vitamin D receptor polymorphism in comparison to healthy control.

Study Type

OBSERVATIONAL

Enrollment

262

Conditions

Keratoconus

Interventions

ELISA,DNA extractionDIAGNOSTIC_TEST

quantitative measurement of (Thyroid stimulating hormon (TSH), free triiodothyronine (FT3)\& Free tetraiodothyronine(FT4)) measured using ELISA . Serum 25-OH vitamin D measured using ELISA. Serum 25-OH vitamin D will be classified based on 2012 American Endocrine Society guidelines into deficient (\< 20ng/ml), insufficient ( 21 -30 ng/ml) and sufficient (\> 30 ng/ml).vitamin D receptor polymorphisms was done in 3 steps: a) DNA extraction from whole blood using Gene Jet gene DNA Purification kits . b) PCR: three Vitamin D receptor polymorphisms were tested \[Taq I (restriction enzymes according to single nucleotide polymorphism ) (rs731236), Apa I (rs7975232) and Bsm I (rs1544410)\] using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Genomic DNA was amplified using certain primers

Eligibility

Sex: ALLMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * keratoconus patients without previously diagnosed thyroid disorder in comparison to healthy control without known systemic disease and with normal cornea who were selected from candidates of refractive surgery with normal topography Exclusion Criteria: * patients who could not provide informed consent or the necessary samples for any reason, patients with associated other ophthalmic pathologies or systemic disorders were excluded from the study

Locations (1)

Eman Azmy

Al Mansurah, Dakahlia Governorate, Egypt

Outcomes

Primary Outcomes

concentration of free T3

pg/ml

Time frame: 2month

concentration of free T4

ng/dl

Time frame: 2 month

concentration of TSH

milli-international units per liter

Time frame: 2 month

vitamin D receptor polymorphism

number of participant with vitamin D receptor polymorphism

Time frame: 2 month

Secondary Outcomes

serum vit D

concentration of Serum 25 OH vitamin D (ng /ml)

Time frame: 2 month

Data from ClinicalTrials.gov

This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.