CRAST is to analyze the expression pattern of circular RNA (circRNA) by bioinformatics analysis in patients with acute ischemic stroke and healthy control. The candidate circRNA will be verified as biomarkers for the detection and prognosis of acute ischemic stroke.
Circular RNAs have been highlighted to be involved in the pathological process of ischemic stroke (IS). The purpose of this protocol will investigate the expression pattern of circular RNA (circRNA) by bioinformatics analysis in patients with acute ischemic stroke and healthy control. The candidate circRNA will be verified as biomarkers for the detection and prognosis of acute ischemic stroke. Distinctive expression patterns of circRNA will be identified by individual quantitative real time polymerase chain reaction (qRT-PCR). A diagnostic or predictive model will be established using logistic regression. The panel of these altered circRNAs may be associated with acute IS and could serve as a novel diagnostic or predictive method.
Study Type
OBSERVATIONAL
Enrollment
500
Quantitative Real-time polymerase chain reaction of circular RNA (circRNA).
Department of Neurology
Xuzhou, Jiangsu, China
RECRUITINGDifferential expression pattern of circRNA
Differential expression pattern of circular RNA (circRNA) will be compared between AIS group and HC group, thus candidate circRNA will be verified as biomarkers for the detection and prognosis of AIS.
Time frame: 90 days
Dynamic changes of circRNA during the follow-up period
Dynamic changes of circRNA will be explored before and after endovascular therapy
Time frame: 90 days
Correlation of circRNA and stroke-associated infection
Correlation of circRNA and stroke-associated infection will be explored in acute ischemic stroke.
Time frame: 30 days
Correlation of circRNA and inflammatory factors in acute ischemic stroke
Correlation of circRNA and inflammatory factors on admission will be explored in acute ischemic stroke.
Time frame: 7 days
Prognostic value of circRNA in acute ischemic stroke
The prognosis of AIS patients will be analyzed between differential expressed candidate circRNA after they have completed the systematic therapy.
Time frame: 90 days
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