Comparing Polydioxanone Thread Embedding Acupuncture and Manual Acupuncture for Nasolabial Fold Reduction

Inclusion Criteria: * Woman * Age 30-50 years old * WSRS scale 3 to 4 * Willing to follow the research to completion * Subjects who do not have a history of filler injection and/implants, laser therapy, microdermabrasion, peeling, botulinum toxin therapy, manual acupuncture on the face, thread embedding acupuncture on the face and does not either oral or topically use collagen within 6 months * Body mass index of ≥ 18.5 * Signed the informed consent Exclusion Criteria: * Subjects with facial muscle paralysis disorders * Subjects with history of keloids and/or hypertrophic scars, subjects with tumors or infection or inflammation at the treatment area * Subjects with allergies to stainless steel, PDO threads, topical anesthetics lidocaine * Pregnant and lactating subjects * Subjects with cancer * Subjects with history of bleeding disorders or are taking anticoagulant or antiplatelet. * Subjects suffering from fever (≥ 37.5°C), cough, fatigue * Subjects with blood glucose POCT (Point of Care Testing) levels of ≥ 200 mg/dl