Effect of Passive Ultrasonic Irrigation on Bacteria That Persist After Root Canal Preparation

University of Sao Paulo30 enrolled

Overview

This study aims to evaluate the effectiveness of ultrasonic irrigation in improving root canal disinfection after chemomechanical procedures.

Although chemomechanical procedures promote a drastic bacterial reduction, many root canals remain infected, which points to the need for complementary procedures to improve root canal disinfection. Thus, this clinical study aims to evaluate the effect of passive ultrasonic irrigation as a supplementary disinfection procedure after root canal preparation. Microbiological samples of the root canals of 30 single-rooted teeth with apical periodontitis will be taken at different stages of the endodontic treatment: before preparation (S1), after chemomechanical procedures (S2) and after passive ultrasonic irrigation (S3). The samples will be submitted to the quantitative polymerase chain reaction for bacterial quantification and to the reverse transcription quantitative polymerase chain reaction for bacterial activity analysis. Data will be analyzed using the Wilcoxon test for paired samples (p \< 0.05).

Study Type

INTERVENTIONAL

Allocation

Purpose

TREATMENT

Masking

NONE

Enrollment

Conditions

Bacterial Infections

Interventions

Ultrasonic irrigationPROCEDURE

Ultrasonic irrigation will be used as a complementary step to chemomechanical procedures to test whether it can improve root canal disinfection.

Eligibility

Sex: ALLMin age: 18 YearsMax age: 65 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Teeth with necrotic pulps and asymptomatic apical periodontitis Exclusion Criteria: * patients who had received antibiotics during the previous 3 months or had any general disease, * teeth that could not be properly isolated with rubber dam, * non-restored teeth, * periodontal pockets depths greater than 4 mm, * previous endodontic treatment, * open apex, * crown/root fracture * root resorption or calcifications.

Locations (1)

University of São Paulo

São Paulo, São Paulo, Brazil

Outcomes

Primary Outcomes

Bacterial Activity

The metabolic activity of total bacteria in root canal samples after ultrasonic irrigation

Time frame: through study completion, an average of 3 years

Secondary Outcomes

Bacterial Levels

Quantitative data of total bacteria determined by quantitative polymerase chain reaction after endodontic procedures

Time frame: through study completion, an average of 3 years

Data from ClinicalTrials.gov

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