Reversal of Aspirin Antiplatelet Therapy With Cold Stored Platelets

Inclusion Criteria: * Subject feels healthy and well * 18-59 years old * All sexes * Temperature \<99.6 F * Resting blood pressure: systolic \<181 mmHg, diastolic \<101 mmHg * Resting heart rate: 40 - 100 beats per minute * Weight: \>109 lb * Hematocrit: \>34% for female subjects, \>37% for male subjects. \<56% for all subjects. * Platelet count able to achieve target platelet yield per apheresis machine configuration parameters * Subjects must be able to read, understand and sign the informed consent document and commit to the study follow-up schedule. The ability to read and speak English is required for participation * Subject must agree to avoid taking any aspirin or aspirin-containing drugs (e.g., Alka-Seltzer®, Bufferin®, Excedrin®) or NSAIDs (e.g.,Feldene®, Motrin®, Aleve®, Advil®) or other drugs known to significantly affect platelet function throughout their study participation determined by PI * Subjects must have "good veins" for apheresis platelet collection and drawing blood samples * Women of child-bearing potential must agree to use an effective method of contraception during the course of the study. The following methods of contraception will be considered 'effective' when self-reported by subject; abstinence, intrauterine contraception devices, hormonal methods, barrier methods or history of sterilization * Subject has phone and e-mail for contact and notification, and is able to come to the research site for study visits Exclusion Criteria: 1. Active acute infection or suspected active infection or taking antibiotics. 2. Active immune/inflammatory condition (e.g. gout, systemic lupus erythematosus, allograft rejection). 3. History of heart disease, including endorsement of shortness of breath with mild exertion (at the discretion of the PI). 4. History of significant liver, kidney, GI, blood, endocrine/metabolic, autoimmune or pulmonary disease, untreated hypertension and or metabolic syndrome (at the discretion of the PI). 5. Diabetes Mellitus. 6. Cancer of any kind (exceptions being basal or squamous cell cancers of the skin), under treatment or resolved. 7. History of bleeding events, family history of bleeding events, or known genetic disorder with bleeding diathesis. 8. A family history of venous or arterial thrombosis before the age of 50 in first degree relatives. 9. A personal history of DVT, venous or arterial thrombosis, blood clots or stroke. 10. History of or currently prescribed antiplatelet therapies \[such as clopidogrel (Plavix), ticlopidine (Ticlid) or Ticagrelor (Brilinta)\] and/or anticoagulant therapies \[such as heparin, enoxaparin (Lovenox), warfarin (Coumadin), apixaban (Eliquis), dabigatran (Pradaxa), edoxaban (Savaysa), rivaroxaban (Xarelto)\]. 11. Subject has taken any aspirin or aspirin-containing drugs (e.g., Alka-Seltzer®, Bufferin®, Excedrin®) or NSAIDs (e.g.,Feldene®, Motrin®, Aleve®, Advil®) or other drugs known to significantly affect platelet function within 14 days prior to screening as determined by PI. 12. Chronic NSAID therapy. 13. Chronic steroid therapy. 14. Known allergy to aspirin. 15. Current drug or alcohol dependence by subject's declaration. 16. Currently pregnant or nursing as assessed during interview. A urine pregnancy test prior to apheresis is required for women of childbearing potential. 17. Subject plans to participate in contact sports during study/observational period such as boxing, rugby, American football, soccer, or other risky recreational hobbies at the discretion of the investigator. 18. Unwilling or unable to comply with the protocol in the opinion of the investigator. 19. Participation in an experimental drug/device study within the past 30 days (other than this study). Subjects who have received an infusion on this study may not be re-enrolled. 20. Other unspecified reasons that, in the opinion of the Investigator, make the subject unsuitable for enrollment