The Age-related Changes of Energy Metabolism in Physically Fit Adult Males

Poznan University of Physical Education420 enrolled

Overview

The influence of age and physical fitness level on the level of energy metabolism and antioxidant capacity in adult males.

Over four hundred healthy, adult males of different age categories and physical fitness levels were included in the study. There is much research convincing that in athletes of various sports disciplines the level of energy metabolism, oxidative stress, and amino acid profiles during years of training undergoes sports adaptation. The study aimed to evaluate how age and physical fitness level affects the level of purine metabolites and metabolites of nicotinamide adenine dinucleotide, the level of antioxidant capacity, and amino acid profiles. Investigators hypothesized that there will be an age-related decline in most of the physiological indices, but significant differences will appear regarding the level of an individual's sports adaptation.

Study Type

INTERVENTIONAL

Allocation

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

420

Conditions

Healthy

Interventions

Cardio-pulmonary exercise testDIAGNOSTIC_TEST

An incremental running test was conducted on the treadmill. Initially, the exercise protocol included standing still for 3 min and walking for 3 min at a constant speed of 4 km/h. After this introductory part, the speed was increased to 8 km/h and then every 3 min by 2 km/h until the volitional exhaustion was reached. During all procedure oxygen consumption was measured with the use of a portable breath-by-breath ergospirometer.

Eligibility

Sex: MALEMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: 1. healthy 2. non-smoking 3. physically fit, including top-level athletes Exclusion Criteria: 1. no reported history of a cardiopulmonary or renal diseases, and other chronic diseases 2. no major orthopedic injury or illness resulting in an inability to run 3. no medications that could affect cardiopulmonary functions 4. normal resting electrocardiogram 5. body mass index (BMI) below 30.0 kg·m-2 6. normal erythrocyte count and Hb content 7. no pathological states known of significantly elevated adenylate pool and concentration of ATP, e.g. sickle cell disease, diabetes, leukemia, sepsis, tuberculosis, meningococcal infection, or renal insufficiency

Locations (1)

Poznan University of Physical Education

Poznan, Poland

Outcomes

Primary Outcomes

Cardio-respiratory measures

Cardiopulmonary exercise test (CPET) was conducted while an incremental running test on the treadmill using a portable breath-by-breath ergospirometer and heart rate monitor.

Time frame: during the intervention

Secondary Outcomes

Blood Lactate level

Lactate concentration was performed by the spectrophotometric enzymatic method.

Time frame: baseline and immediately after the intervention

Plasma purine nucleotides

The plasma purine metabolites were estimated in plasma using high-performance liquid chromatography (HPLC) with UV-VIS detection. Measurements performed: Guanosine, Inosine, Adenosine, Hypoxanthine, Xanthine, Uric Acid, Allantoin, Adenine Nucleotides \[adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosine monophasphate (IMP)\], Guanine Nucleotides \[guanosine triphosphate (GTP), guanosine diphosphate (GDP), guanosine monophosphate (GMP)\].

Time frame: baseline and immediately after the intervention

Erythrocyte Purine Metabolites

The erythrocyte purine metabolites were estimated in erythrocytes using high-performance liquid chromatography (HPLC) with UV-VIS detection. Measurements performed: Adenine Nucleotides \[adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), inosine monophasphate (IMP)\], Guanine Nucleotides \[guanosine triphosphate (GTP), guanosine diphosphate (GDP), guanosine monophosphate (GMP)\].

Time frame: baseline and immediately after the intervention

Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) activity

The HGPRT activity was estimated in whole blood and in erythrocytes using high-performance liquid chromatography (HPLC) with UV-VIS detection.

Time frame: baseline and immediately after the intervention

Data from ClinicalTrials.gov

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