Human Auto-ethanol Formation and Acetate Signaling

Subjects will be recruited to the trial in two seasons. First, eight healthy men and post-menopausal women 50-75 years will be recruited for the pre-trial during autumn/winter 2021. Secondly, 16 healthy men and women with an age-range of 25-75 years will be recruited for the main trial during late spring/summer 2022. Age-specific inclusion criteria for the pre-trial: * Healthy men and healthy, post-menopausal women (12 consecutive months without menstrual period) * Age: 50-75 years old * BP ≤ 140/90 (systolic/diastolic) * Plasma glucose \<7 mmol/l * BMI: 18.5-27 kg/m2 * Subjects with a good command of both written and verbal English Age-specific inclusion criteria for the main-trial: * Healthy men and women * Age: 25-75 years old * BP ≤ 140/90 (systolic/diastolic) * Plasma glucose \<7 mmol/l * BMI: 18.5-27 kg/m2 Inclusion criteria for the pre- and main-trial: * Willing to provide registration of compliance during the run-in period and follow-up periods * Willing to avoid drinking alcohol for 2 weeks before the first test day and between test days * Willing to stay in the respiration chamber the night before and after each test day * Owning a smartphone Exclusion Criteria: * Alcohol naïve or has not consumed alcohol within the last year * Any history of alcohol or substance abuse or a high alcohol intake, defined as: * An Alcohol Use Disorders Identification Test (AUDIT, appendix 1) score \> 5 at screening * Drinking on average \>14 alcoholic beverages a week during the past 6 months * Yale-Brown Obsessive Compulsive Scale-heavy drinking (Y-BOCS-hd, appendix 2) total score of ≥6 on questions 1, 2, and 3 * Intolerance or allergy to alcoholic beverages, juniper or citrus * Diagnosed with any CVD event (MI, revascularization procedure or stroke) within the past six months * Diagnosed with any known or past severe chronic disease including liver diseases (e.g., active hepatitis B and C infections, liver cirrhosis, hepatitis, cancer), T2DM, prediabetes, hypertension, severe psychiatric illness or frequent use of medication (except over-the-counter drugs or mild anti-depressants) or any other clinical condition that makes the subject ineligible according to the clinically responsible medical doctor (self-reported). * Cancer - active malignant cancer or history of malignancy within the last 5 years (with exception of non-melanoma skin cancer). * Previous breast cancer diagnosis or at high risk of breast cancer defined as: * Breast Cancer Risk Assessment Tool (BCRAT) risk score \> 5 % (https://bcrisktool.cancer.gov/calculator.html) * Close relatives with diagnosed breast cancer (mother, sister, daughter) * A Patient Health Questionnaire (PHQ-9, appendix 3) ≥15 at screening or a positive response on question 9 (thoughts about suicide) * Diagnosed with atrial fibrillation * Hemoglobin (Hb) levels below 7.3 mmol/L for women and 8.3 mmol/L for men. * Chronic use of any type of medication, except for mild antidepressants or contraceptives * Any use of contraindicated medication for alcohol intake, such as disulfiram, dual antiplatelet therapy, metronidazole, warfarin or hormone replacement therapy * Hypersensitive to plasters * Use of any type of antibiotics within two month before the first test day * Unintentional weight loss \>20% during the last 6 months * Any type of gastrointestinal problems or prior surgery expected to influence gut health and absorption. * Estimated glomerular filtration rate (eGFR) \< 30 ml/min/1.73 m2 or end-stage renal disease * Liver function tests \>2 times the upper limit of the normal range according to current limits at "sundhed.dk": alanine transaminase (ALT), aspartate aminotransferase (AST), and gamma-glutamyltransferase (GGT). * Blood donations within the past three months before the recruitment * Having no hair on the head (bald-headed) * Current participation in another trial * Not willing to sign the Informed consent form (ICF) * Not willing to comply with the all the trial procedures including completion of the two-week run-in periods without drinking alcohol as well the two-week wash-out periods * Unable or unwilling to follow the safety procedures related to Covid19 * Any other issue that makes the project responsible researcher doubt the eligibility of the volunteer