Blocking histamine H1/H2 receptors blunts chronic endurance training adaptations. The current study addresses a twofold research question: "What is the influence of endurance training (1) and histamine H1 and H2 signaling (2) on the gene expression in human skeletal muscle." Results from this study will yield more insights into the molecular mechanisms of adaptations to exercise training.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
TRIPLE
Enrollment
14
Oral placebo Exercise training: Interval-based cycling exercise
H1 blockade: oral blockade Exercise training: Interval-based cycling exercise
H2 blockade: oral blockade Exercise training: Interval-based cycling exercise
Department of movement and sports sciences, Ghent University, Belgium
Ghent, Oost-Vlaanderen, Belgium
Change in muscle transcriptome (direct response)
RNA-sequencing
Time frame: On each test-day: 0 minutes after the exercise training versus at rest.
Change in muscle transcriptome (delayed response)
RNA-sequencing
Time frame: On each test-day: 180 minutes after the exercise training versus at rest.
Muscle glycogen depletion
Fluorometric determination of muscle glycogen levels
Time frame: On each test-day: 0 minutes after the exercise training versus at rest.
Muscle glycogen resynthesis
Fluorometric determination of muscle glycogen levels
Time frame: On each test-day: 180 minutes after the exercise training versus at rest.
Plasma volume change
Plasma volume based on hemoglobin and hematocrit concentration.
Time frame: Change from rest to different time-points after exercise (0, 30, 60, 120 and 180 minutes after the exercise training).
Heart rate during the exercise training
Heart rate during the exercise training.
Time frame: Continuously during training on each test-day.
Blood lactate
Capillary lactate concentration.
Time frame: On each test-day at 10 time-points: at rest, during exercise and 0, 30, 60, 120 and 180 minutes after the exercise training.
Blood glucose
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.
Capillary glucose concentration.
Time frame: On each test-day at 10 time-points: at rest, during exercise and 0, 30, 60, 120 and 180 minutes after the exercise training.
Blood histamine
histamine concentration in blood samples.
Time frame: On each test-day at 6 time-points: at rest and 0, 30, 60, 120 and 180 minutes after the exercise training.
Blood insulin
insulin concentration in blood samples.
Time frame: On each test-day at 6 time-points: at rest and 0, 30, 60, 120 and 180 minutes after the exercise training.
Muscle signaling pathways relevant for glucose metabolism and cardiovascular health
Phosphorylation status of proteins assessed by Western Blotting
Time frame: On each test-day: at rest, 0 minutes and 180 minutes after the exercise training.