The aim of this study was to investigate the therapeutic effect of co-administration of pentoxifylline and zinc on sperm apoptosis in men with idiopathic infertility
This study is being performed as a double-blind randomized clinical trial on idiopathic infertile men referred to the infertility clinic of Shafa Clinic. Patient satisfaction, non-use of contraceptives, men aged 25 to 43 years, and idiopathic infertility according to WHO criteria (World Health, 2010) are our study criteria. Any medication that may affect the course of spermatogenesis should be discontinued during the study. Semen samples are taken from patients (before and after interventions) and apoptosis tests are performed on semen samples.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
DOUBLE
Enrollment
160
Zinc is an element that is involved in the activity of antioxidant enzymes. For example, the superoxide dismutase needs copper and zinc to function properly. Infertile men with asthenospermia receive 220 mg zinc sulfate tablets for 3 months
Oral use of pentoxifylline is effective in treating cardiovascular disease, cerebrovascular disease, and other conditions associated with local circulatory disorders. Pentoxifylline affects blood viscosity. Infertile men with asthenospermia receive 400 mg pentoxifylline tablets for 3 months
Infertility Center
Arak, Markazi Province, Iran
RECRUITINGArak university of medical science
Arak, Markazi Province, Iran
COMPLETEDsperm concentration
count of sperm(In per million - with the help of a neobar slide and microscopic observation
Time frame: 1 day
spem motility
motility of sperm: Calculate the percentage of motile sperm using microscopic observation
Time frame: 1 day
sperm morphology
Calculate the percentage of sperm with a normal shape with the help of Papanic staining
Time frame: 1 day
lipid proxidation
Malondialdehyde (nmol/mL), is measured using related experimental kits
Time frame: One week
reactive oxygen species
eactive oxygen species (ROS (RLU/s)), is measured using related experimental kits
Time frame: One week
total antioxidant capacity
total antioxidant capacity (TAC (/m / l)) is measured using related experimental kits
Time frame: One week
FSH hormon
FSH (IU / L) is measured from the blood serum of volunteers using ELISA kit
Time frame: One week
LH hormon
LH (IU / L) is measured from the blood serum of volunteers using ELISA kit
Time frame: One week
testosteron hormon [ Time Frame: One week after starting the medication ] testosterone
testosterone (nanomolar per liter) is measured from the blood serum of volunteers using ELISA kit
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Time frame: One week
(Tissue necrosis Factor)
TNF α( Tissue necrosis Factor) (pg/mL) as an inflammatory factor is measured using related experimental kit
Time frame: One week
interleukin-6
interleukin-6 (IL-6)/(pg/mL) as an inflammatory factor is measured using related experimental kit
Time frame: One week
Sperm DNA Fragmentation Assay(SDFA)
DNA fragmentation is assessed using an SDFA kit and microscopic observations (as a percentage of damaged sperm).
Time frame: One week
BAX expression
BAX expression \[ Time Frame: 3 months \] expression of BAX gen(in p.c) using real-time PCR and Western blot
Time frame: 3 month
Bcl2 expression
Bcl-2 gen(in p.c) using real-time PCR and Western blot
Time frame: 3 months
caspase 3 expression
expression of caspase 3 gen(in p.c) using real-time PCR and Western blot
Time frame: 3 months