Comparison of the Genotoxicity of Desflurane and Propofol

Muğla Sıtkı Koçman University30 enrolled

Overview

The purpose of this study is to evaluate if the anesthetics propofol and desflurane can damage DNA according to comet essay in patients submitted to lumbar disc surgery.

It is expected that DNA damage information provide the possible genotoxic effects of inhalational and intravenous anesthetics and contribute to the establishment of strategies aimed at reducing the risks to exposed patients.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

DIAGNOSTIC

Masking

DOUBLE

Enrollment

Conditions

DNA Damage Anesthesia

Interventions

Anesthetics, General Desflurane MaintenanceDRUG

Comparison of dna damage of desflurane maintenance with propofol maintenance according to comet analysis

Anesthetics, General Propofol MaintenanceDRUG

Comparison of dna damage of propofol maintenance with desflurane maintenance according to comet analysis

Eligibility

Sex: ALLMin age: 18 YearsMax age: 65 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: 1. Ability to understand and willingness to sign an informed consent form 2. Elective lumbar spinal surgery that will take at least 120 minutes, 3. American Society of Anesthesiologists (ASA) physical status I and II Exclusion Criteria: 1. Patients with ASA physical status III or IV disease 2. Chronic systemic disease (diabetes mellitus,chronic pulmonary disorders, chronic kidney and liver disorders) 3. Smoking or alcohol consumption 4. Recently received radiation, chemotherapy 5. Malignancy 6. Patients who will receive blood transfusion during the operation 7. Users of antioxidant supplements 8. Patients who have received general anesthesia in the last three months 9. Patients with known occupational exposures (operating room personnel, chemical plant worker

Locations (1)

Mugla Sitki Kocman University

Muğla, Turkey (Türkiye)

Outcomes

Primary Outcomes

Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire

The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage.

Time frame: Before anesthesia induction

Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire

The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage.

Time frame: 2 hours after anesthesia induction

Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire

The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage.

Time frame: postoperative 1st day

Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire

The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage.

Time frame: postoperative 5th day

Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire

The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage.

Time frame: Before anesthesia induction

Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire

The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage.

Time frame: 2 hours after anesthesia induction

Data from ClinicalTrials.gov

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