This subproject aims to evaluate a new nerve excitability protocol to induce itch in healthy controls by electrical stimulation. Itch has many similarities with pain, and the nerve fibers that mediate the two sensations probably have the same morphological and excitability properties. The method used in this project will be the same as our research group uses for studying nociceptor fibers (PTT technique), but the focus is on the itch sensation. Since the itch fibers also terminate in the epidermis, our PTT technique should also be able to activate itch fibers, similar to pain fibers. If itch could be effectively induced by electrical stimulation, this would generate a valuable tool for studying itch in healthy controls. The sub-project takes place in 2 sessions (2 hours each).
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
20
At the start of session 1, one area of the middle forearms of the participant will be located (4x4cm). On the area, anti-histamine cream will be applied for approximately 1 hour.
At the start of session 2, one area of the middle forearms of the participant will be located (4x4cm). On the area, local anesthetic cream (2.5% lidocain/2.5 prilocaine) will be applied for approximately 1 hour.
Aalborg University
Aalborg, Aalborg Ø, Denmark
Perception threshold for pulse shape 1
The perception threshold will be estimated for the electrical stimuli with pulse shape 1. The perception threshold is defined as the transcutaneous electrical current needed for the participant to perceive the stimulus. The electrode will be placed on the arm. The perception threshold will be estimated by the method of limits and the psi method.
Time frame: This outcome measurement will be estimated during sessions one and two. The outcome measure takes approximately 5 minutes to estimate.
Perception threshold for pulse shape 2
The perception threshold will be estimated for the electrical stimuli with pulse shape 2. The perception threshold is defined as the transcutaneous electrical current needed for the participant to perceive the stimulus. The electrode will be placed on the arm. The perception threshold will be estimated by the method of limits and the psi method.
Time frame: This outcome measurement will be estimated during sessions one and two. The outcome measure takes approximately 5 minutes to estimate.
Perception threshold for pulse shape 3
The perception threshold will be estimated for the electrical stimuli with pulse shape 3. The perception threshold is defined as the transcutaneous electrical current needed for the participant to perceive the stimulus. The electrode will be placed on the arm. The perception threshold will be estimated by the method of limits and the psi method.
Time frame: This outcome measurement will be estimated during sessions one and two. The outcome measure takes approximately 5 minutes to estimate.
Perception threshold for pulse shape 4
The perception threshold will be estimated for the electrical stimuli with pulse shape 4. The perception threshold is defined as the transcutaneous electrical current needed for the participant to perceive the stimulus. The electrode will be placed on the arm. The perception threshold will be estimated by the method of limits and the psi method.
Time frame: This outcome measurement will be estimated during sessions one and two. The outcome measure takes approximately 5 minutes to estimate.
Perception threshold for pulse shape 1 when antihistamine cream has been applied
The perception threshold will be estimated for the electrical stimuli with pulse shape 1 when anti-histamine cream has been applied for approximately an hour. The perception threshold is defined as the transcutaneous electrical current needed for the participant to perceive the stimulus. The electrode will be placed on the arm. The perception threshold will be estimated by the method of limits and the psi method.
Time frame: This outcome measurement will be estimated during session one. The outcome measure takes approximately 5 minutes to estimate.
Perception threshold for pulse shape 2 when anti-histamine cream has been applied
The perception threshold will be estimated for the electrical stimuli with pulse shape 2 when anti-histamine cream has been applied for approximately an hour. The perception threshold is defined as the transcutaneous electrical current needed for the participant to perceive the stimulus. The electrode will be placed on the arm. The perception threshold will be estimated by the method of limits and the psi method.
Time frame: This outcome measurement will be estimated during session one. The outcome measure takes approximately 5 minutes to estimate.
Perception threshold for pulse shape 3 when anti-histamine cream has been applied
The perception threshold will be estimated for the electrical stimuli with pulse shape 3 when anti-histamine cream has been applied for approximately an hour. The perception threshold is defined as the transcutaneous electrical current needed for the participant to perceive the stimulus. The electrode will be placed on the arm. The perception threshold will be estimated by the method of limits and the psi method.
Time frame: This outcome measurement will be estimated during session one. The outcome measure takes approximately 5 minutes to estimate.
Perception threshold for pulse shape 4 when anti-histamine cream has been applied
The perception threshold will be estimated for the electrical stimuli with pulse shape 4 when anti-histamine cream has been applied for approximately an hour. The perception threshold is defined as the transcutaneous electrical current needed for the participant to perceive the stimulus. The electrode will be placed on the arm. The perception threshold will be estimated by the method of limits and the psi method.
Time frame: This outcome measurement will be estimated during session one. The outcome measure takes approximately 5 minutes to estimate.
Perception threshold for pulse shape 1 when local anaesthetic cream has been applied
The perception threshold will be estimated for the electrical stimuli with pulse shape 1 when local anaesthetic cream has been applied for approximately an hour. The perception threshold is defined as the transcutaneous electrical current needed for the participant to perceive the stimulus. The electrode will be placed on the arm. The perception threshold will be estimated by the method of limits and the psi method.
Time frame: This outcome measurement will be estimated during session two. The outcome measure takes approximately 5 minutes to estimate.
Perception threshold for pulse shape 2 when local anaesthetic cream has been applied
The perception threshold will be estimated for the electrical stimuli with pulse shape 2 when local anaesthetic cream has been applied for approximately an hour. The perception threshold is defined as the transcutaneous electrical current needed for the participant to perceive the stimulus. The electrode will be placed on the arm. The perception threshold will be estimated by the method of limits and the psi method.
Time frame: This outcome measurement will be estimated during session two. The outcome measure takes approximately 5 minutes to estimate.
Perception threshold for pulse shape 3 when local anaesthetic cream has been applied
The perception threshold will be estimated for the electrical stimuli with pulse shape 3 when local anaesthetic cream has been applied for approximately an hour. The perception threshold is defined as the transcutaneous electrical current needed for the participant to perceive the stimulus. The electrode will be placed on the arm. The perception threshold will be estimated by the method of limits and the psi method.
Time frame: This outcome measurement will be estimated during session two. The outcome measure takes approximately 5 minutes to estimate.
Perception threshold for pulse shape 4 when local anaesthetic cream has been applied
The perception threshold will be estimated for the electrical stimuli with pulse shape 4 when local anaesthetic cream has been applied for approximately an hour. The perception threshold is defined as the transcutaneous electrical current needed for the participant to perceive the stimulus. The electrode will be placed on the arm. The perception threshold will be estimated by the method of limits and the psi method.
Time frame: This outcome measurement will be estimated during session two. The outcome measure takes approximately 5 minutes to estimate.
Sensation for pulse 1
The investigator will ask the participant to rate the sensation of electrical stimulation with pulse 1 for the three different conditions. The conditions are: control (not active cream), antihistamine cream, and local anesthetic cream. The scale is 0-10 for the three sensations touch, itch, and pain. Zero means no sensation, and ten is the worst pain/touch/itch imaginable.
Time frame: This outcome measurement will be estimated during sessions one and two. The outcome measure takes approximately 5 minutes to estimate.
Sensation for pulse 2
The investigator will ask the participant to rate the sensation of electrical stimulation with pulse 2 for the three different conditions. The conditions are: control (not active cream), antihistamine cream, and local anesthetic cream. The scale is 0-10 for the three sensations touch, itch, and pain. Zero means no sensation, and ten is the worst pain/touch/itch imaginable.
Time frame: This outcome measurement will be estimated during sessions one and two. The outcome measure takes approximately 5 minutes to estimate.
Sensation for pulse 3
The investigator will ask the participant to rate the sensation of electrical stimulation with pulse 3 for the three different conditions. The conditions are: control (not active cream), antihistamine cream, and local anesthetic cream. The scale is 0-10 for the three sensations touch, itch, and pain. Zero means no sensation, and ten is the worst pain/touch/itch imaginable.
Time frame: This outcome measurement will be estimated during sessions one and two. The outcome measure takes approximately 5 minutes to estimate.
Sensation for pulse 4
The investigator will ask the participant to rate the sensation of electrical stimulation with pulse 4 for the three different conditions. The conditions are: control (not active cream), antihistamine cream, and local anesthetic cream. The scale is 0-10 for the three sensations touch, itch, and pain. Zero means no sensation, and ten is the worst pain/touch/itch imaginable.
Time frame: This outcome measurement will be estimated during sessions one and two. The outcome measure takes approximately 5 minutes to estimate.
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