SAD/MAD Safety and PK Study of RBN-3143 in Healthy and Atopic Dermatitis Subjects

Inclusion Criteria: 1. Healthy male or female subjects between 18 and 65 years of age (inclusive at the time of informed consent) 2. Body mass index (BMI) between ≥18 and ≤30 kg/m2 (inclusive) at Screening 3. Good general health, with no significant medical history, have no clinically significant abnormalities on physical examination at Screening and/or before administration of the initial dose of study drug 4. Clinical laboratory values within normal range as specified by the testing laboratory, unless deemed not clinically significant by the Investigator 5. Willingness and ability to speak, read, and understand English, and provide written informed consent 6. Must be a non-smoker or former smoker. Subjects must have negative cotinine results in drug tests at Screening and Baseline 7. Females must be: Non-pregnant Non-lactating Must use a non-hormonal, acceptable, highly effective double contraception from Screening until study completion, including the Follow-up Period Acceptable non-hormonal double contraception is defined as a condom AND one other form of the following: * An IUD (non-hormonal) * Documented evidence of surgical sterilization at least 6 months prior to Screening (e.g., tubal occlusion, hysterectomy, bilateral salpingectomy, or bilateral oophorectomy for women or vasectomy for men \[with appropriate post-vasectomy documentation of the absence of sperm in semen\] provided the male partner is a sole partner) Women of child-baring potential (WOCBP) must have: A negative pregnancy test at Screening and Day -1 and be willing to have additional pregnancy tests as required throughout the study Post-menopausal Women: Women not of childbearing potential must be: Postmenopausal for ≥12 months Postmenopausal status will be confirmed through testing of FSH levels ≥ 40 IU/L at Screening for amenorrhoeic female subjects Abstinence: Females who are abstinent from heterosexual intercourse will also be eligible. Complete abstinence by the subject for the duration of the study and for 1 month after the last study treatment is acceptable. Note: Periodic abstinence (eg, calendar, ovulation, symptothermal, post ovulation methods) and withdrawal are not considered highly effective methods of birth control. Female subjects who are in same-sex relationships are not required to use contraception. Males: If engaged in sexual relations with a WOCBP: The subject or his partner must be surgically sterile (eg, \>30 days since vasectomy with no viable sperm, tubal occlusion, hysterectomy, bilateral salpingectomy, bilateral oophorectomy) Must use an acceptable, highly effective contraceptive method from Screening until study completion, including the Follow-up Period. Acceptable methods of contraception include the use of condoms and the use of an effective contraceptive for the female partner that includes: * OCPs * Long acting implantable hormones * Injectable hormones * Vaginal ring * Use of an IUD Subjects with same-sex partners (abstinence from penile-vaginal intercourse) are eligible 8. For at least 90 days after the last dose of study drug, male subjects must not donate sperm and female subjects must not donate ovum 9. Willingness to comply with the drawing of all blood samples for the PK/PD analysis. 10. Willing and able to attend the necessary visits to the CRU and comply with all testing, fasting, and requirements defined in the protocol. 11. Willing and able to remain at the study site unite for the duration of the confinement period and return for outpatient visit/s defined in the protocol. FOR PART B (Moderate/Severe AD COHORT ONLY) In addition to the inclusion criteria stated above, patients must meet the following criteria for inclusion in Part B: 12. Chronic atopic dermatitis (AD) diagnosed by the Eichenfield revised criteria of Hanifin and Rajka, that has been present for at least 6 months before the Screening visit 13. EASI score ≥ 8 at the Screening and Baseline visits 14. IGA score ≥ 3 at the Screening and Baseline visits 15. Equal to or greater than 10% body surface area of AD involvement in areas affected beyond palms and soles at the Screening and Baseline visits, as per SCORAD 16. A documented history by the Investigator or delegate of inadequate response to treatment with topical medications within the past 6 months defined as failure to achieve or maintain remission or mild disease activity state (IGA score ≤ 2) despite daily treatment with topical corticosteroids of medium or higher potency (with or without topical calcineurin inhibitors) applied for at least 4 weeks or for the maximum recommended by the product manufacturer, whichever is shorter. 17. The patient must be applying stable doses of an additive-free, basic bland emollient twice-daily for at least 7 days before the Baseline visit 18. The patient must be willing to comply with skin biopsy/specimen collections Exclusion Criteria: 1. Any medical condition that is considered by the Investigator or delegate that may interfere with study assessments, may adversely affect the subject's participation in the study, may make the subject's participation in the study unreliable, or be of such severity as to present an increased risk to the subject because of participation in the study. 2. Prior or ongoing medical conditions, physical findings, laboratory abnormality or a history of neurological, hepatic, renal, endocrine, cardiovascular, gastrointestinal, pulmonary, or metabolic disease that is considered as significant by the Investigator or delegate, and in the Investigator's opinion, could adversely affect the safety of the subject 3. Abnormal ECG findings at Screening that are considered by the Investigator to be clinically significant: 1. Significant history of cardiovascular disease 2. ECG results showing QTcF \>450 msec or the presence of clinically significant abnormalities as determined by the investigator (Screening or Day -1) 3. Elevation of blood pressure (BP), i.e., supine systolic BP \>140 mmHg and/or diastolic BP \>90 mmHg, or heart rate \>100 beats per minute at rest 4. Use of any prescription medication within 14 days of dosing or over-the-counter (OTC) medication (including vitamins) within 48 hours of dosing or intends to use any prescription medication or OTC medication during the study that may interfere with the evaluation of study medication. Co-administration of medications known to have high risk of prolonging the QT interval are also prohibited. The use of other concomitant medications that present a low risk of QT prolongation may be considered, with the approval of the Medical Monitor. Simple analgesia (paracetamol, nonsteroidal anti-inflammatory drug \[NSAID\]) may be permitted at the discretion of the Investigator or delegate. Use of a low dose corticosteroid and β2 agonist inhalers to treat concomitant Asthma are allowed. 5. Ingestion of herbal medicines within 3 weeks before Screening, and grapefruit, grapefruit juice, star fruit or orange marmalade (made with Seville oranges) within 2 weeks prior to dosing, or intends to use any of these products during the study 6. Relevant dietary restrictions or unwilling to consume standard meals provided. 7. Ingestion of caffeine- or xanthine-containing products (e.g., coffee, tea, cola drinks, and chocolate) on days scheduled for full PK sample collection from 10 hours prior to the start of dosing through 12 hours post-dose. Must refrain from the consumption of alcohol for 2 days prior to Day 1 through the end of the study 8. A history of substance abuse or dependency or history of recreational intravenous (IV) drug use over the last 1 year (by self-declaration); or a positive ethanol breath test, urine cotinine, or urine drug screen at Screening and at Day -1 9. A positive test for hepatitis C antibody (HCV), hepatitis B surface antigen (HBsAg), human immunodeficiency virus (HIV) antibody, or COVID-19 (if conducted, at the Investigator's discretion) at Screening 10. Pregnant or lactating at Screening or planning to become pregnant (self or partner) at any time during the study, including the Follow-up Period 11. Use of any investigational product (IP) or investigational medical device within 30 days prior to Screening, or 5 half-lives of the product (whichever is the longest) or participation in more than 4 investigational drug studies within 1 year prior to Screening 12. Donated or lost a significant volume of blood (\>450 mL) within 4 weeks prior to the first study drug administration 13. Unwilling to reside in the study unit for the duration of the study or to cooperate fully with the Investigator, delegate or site personnel. 14. Alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and/or total bilirubin \>1.5 × upper limit of normal at Screening. Repeat testing at Screening is acceptable for out-of-range values following approval by the Investigator or delegate 15. Estimated Glomerular Filtration Rate (eGFR) \< 90 mL/min/1.73m2 16. Presence of any underlying physical or psychological medical condition that, in the opinion of the Investigator, would make it unlikely that the subject will comply with the protocol or complete the study per protocol 17. Plasma donation within 7 days prior to the first study drug administration 18. Fever (temperature \>38°C) or symptomatic viral or bacterial infection within 2 weeks prior to Screening 19. History of severe allergic or anaphylactic reactions to medicines or vaccines 20. History of malignancy, except for non-melanoma skin cancer, excised more than 2 years ago and cervical intraepithelial neoplasia that has been successfully cured more than 5 years prior to Screening 21. History or presence of a condition associated with significant immunosuppression 22. History of life-threatening infection (e.g., meningitis) 23. Infections requiring parenteral antibiotics within the 6 months prior to Screening 24. For the Food effect cohort only: a diet that in the opinion of the Investigator is incompatible with the on-study diet (e.g., lactose intolerance diet) 25. Unwilling to refrain from strenuous exercise within 48 hours prior to visits and during confinement at the CRU FOR PART B (AD COHORT ONLY) In addition to the exclusion criteria stated above, patients must not enter Part B if any of the following exclusion criteria apply: 26. Use of any IP or investigational medical device within 8 weeks prior to Screening, or participation in more than 2 investigational drug studies within 1 year prior to Screening 27. Estimated Glomerular Filtration Rate (eGFR) \< 60 mL/min/1.73 m2 at Screening 28. Vaccination with a live (attenuated) vaccine within 8 weeks before the Baseline visit 29. Treatment with allergen immunotherapy within 6 months before Screening 30. Treatment with leukotriene inhibitors within 4 weeks before the Baseline visit 31. Treatment with systemic corticosteroids within 4 weeks before the Baseline visit 32. Treatment with topical corticosteroids, tacrolimus, and/or pimecrolimus within 1 week before the Baseline visit 33. Systemic treatment for AD with an immunosuppressive or immunomodulating substance, eg, cyclosporine, mycophenolate-mofetil, IFN-γ, phototherapy (narrow band ultraviolet B \[NBUVB\], ultraviolet B \[UVB\], ultraviolet A1 \[UVA1\], psoralen + ultraviolet A \[PUVA\]), azathioprine, methotrexate, within 4 weeks before the Baseline visit and within 8 weeks for biologics 34. Use of any concomitant medication that is a moderate or strong inhibitor or inducer of CYP3A4 unless stopped 14 days prior to start of study treatment. Please refer to Section 28 (Appendix 1) for examples of such medications. 35. Use of any concomitant medication that is an inhibitor or inducer of P glycoprotein (P-gp) unless stopped 14 days prior to the start of study treatment. Please refer to Section 28 (Appendix 1) for examples of such medications. 36. Use of any concomitant medication that is a sensitive substrate of CYP3A4 and that, if underdosed, would constitute a significant risk to the subject. Individual cases may be discussed with the MM. Please refer to Section 28 (Appendix 1) for examples of such medications. 37. Use of a necessary concomitant medication where the exposure is dependent on drug transporters organic anion transporting polypeptide1B3 (OATP1B3) or multidrug and toxin extrusion protein 1 or 2-K (MATE1, MATE2-K) and that if overdosed would constitute a significant risk to the patient. Individual cases may be discussed with the MM 38. Subjects who have failed prior systemic treatment to selective inhibitor agents that regulate the interleukin signaling pathway (eg, dupilumab, JAK1 inhibitors except for veracitinib) 39. Treatment of AD with active medications (eg, Atopiclair®, MimyX®, Epicerum®, Cerave®, etc) within 1 week before the Baseline visit 40. Chronic or acute infection requiring treatment with oral or IV antibiotics, anti-virals, anti-parasitics, anti-protozoals, or anti-fungals within 4 weeks before the Baseline visit, or superficial skin infections within 1 week before the Baseline visit