1. Through research and analysis, the levels of upstream and downstream factors related to TGF-β/Smad signaling pathway and inflammatory response factor related factors in serum, blood cells, articular fluid, urine, tongue covering and discarded tissues after surgery of patients with LKD syndrome and PBS syndrome of knee osteoarthritis were obtained, and the syndrome characteristics were further obtained. 2. Through research and analysis, the index levels of multiple omics detection in serum, blood cells, joint fluid, urine, tongue coating and discarded tissues of patients with LKD syndrome and PBS syndrome of knee osteoarthritis were obtained, and the syndrome characteristics were further obtained. 3. The severity of syndrome was quantified by syndrome score scale and the correlation between the above results and the syndrome score of patients with knee osteoarthritis with LKD syndrome and PBS syndrome was studied in combination with imaging characteristics. 4. To establish a "disease and syndrome cell model" and test relevant indicators to support the above research. 5. Through the analysis of the above research results, the possible mechanism is analyzed to provide an objective basis for the biological basis of the syndrome and relevant evidence for the clinical diagnosis and treatment of knee osteoarthritis with traditional Chinese medicine.
1. Through research and analysis, the levels of upstream and downstream factors related to TGF-β/Smad signaling pathway and inflammatory response factor related factors in serum, blood cells, articular fluid, urine, tongue covering and discarded tissues after surgery of patients with liver and kidney deficiency syndrome and phlegm and blood stasis syndrome of knee osteoarthritis were obtained, and the syndrome characteristics were further obtained. 2. Through research and analysis, the index levels of multiple omics detection in serum, blood cells, joint fluid, urine, tongue coating and discarded tissues of patients with liver and kidney deficiency syndrome and phlegm and blood stasis syndrome of knee osteoarthritis were obtained, and the syndrome characteristics were further obtained. 3. The severity of syndrome was quantified by syndrome score scale and the correlation between the above results and the syndrome score of patients with knee osteoarthritis with liver and kidney deficiency syndrome and phlegm and blood stasis syndrome was studied in combination with imaging characteristics. 4. To establish a "disease and syndrome cell model" and test relevant indicators to support the above research. 5. Through the analysis of the above research results, the possible mechanism is analyzed to provide an objective basis for the biological basis of the syndrome and relevant evidence for the clinical diagnosis and treatment of knee osteoarthritis with traditional Chinese medicine.
Study Type
OBSERVATIONAL
Enrollment
340
cross-sectional study without intervention
Dongzhimen Hospital
Beijing, Beijing Municipality, China
RECRUITINGthe differences of metabonomics
The differences of metabonomics in blood, urine and tongue coating will be detected by metabonomics in LKD group, PBS group and NC group.
Time frame: 3 years
the differences of metabonomics in the cell models of disease-TCM syndrome
The differences of metabonomics in the cell models of disease-TCM syndrome will be detected by metabonomics.
Time frame: 3 years
the differences of proteomics in the cell models of disease-TCM syndrome
The differences of proteomics in the cell models of disease-TCM syndrome will be detected by proteomics.
Time frame: 3 years
the differences of proteomics
The differences of proteomics in blood, urine and tongue coating will be detected by proteomics in LKD group, PBS group and NC group.
Time frame: 3 years
the differences of transcriptomics
The differences of transcriptomics in blood, urine and tongue coating will be detected by transcriptomics in LKD group, PBS group and NC group.
Time frame: 3 years
the differences of transcriptomics in the cell models of disease-TCM syndrome
The differences of transcriptomics in the cell models of disease-TCM syndrome will be detected by transcriptomics.
Time frame: 3 years
the related indexes of TGF- β/ Smad signaling pathway and inflammatory response
Histological and pathological examination is used to detect the related indexes of TGF- β/ Smad signaling pathway and inflammatory response.
Time frame: 3 years
The clinical TCM scores of LKD
The minimum value is 0 and maximum value is 38, and higher scores mean a worse outcome.
Time frame: 3 years
The clinical TCM scores of PBS
The minimum value is 0 and maximum value is 41, and higher scores mean a worse outcome.
Time frame: 3 years
The grading scores of Kellgren Lawrence (K-L)
The minimum value is 0 and maximum value is 4, and higher scores mean a worse outcome.
Time frame: 3 years
The grading scores of Yulish
The minimum value is 0 and maximum value is 4, and higher scores mean a worse outcome.
Time frame: 3 years
Clinical data
Collect clinical data and formulate a unified questionnaire, including the name, age, gender, outpatient / inpatient number, past history, current medical history, imaging images and other relevant data of patients with knee osteoarthritis.
Time frame: 3 years
Concentration of BLC (CXCL13)
Concentration of BLC (CXCL13) will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of BLC (CXCL13) in the cell models of disease-TCM syndrome
Concentration of BLC (CXCL13) will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of Eotaxin-1 (CCL11)
Concentration of Eotaxin-1 (CCL11) will be detected by ELISA will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of Eotaxin-1 (CCL11) in the cell models of disease-TCM syndrome
Concentration of Eotaxin-1 (CCL11) will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of Eotaxin-2 (MPIF-2/CCL24)
Concentration of Eotaxin-2 (MPIF-2/CCL24) will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of Eotaxin-2 (MPIF-2/CCL24) in the cell models of disease-TCM syndrome
Concentration of Eotaxin-2 (MPIF-2/CCL24) will be detected by ELISA in the cell models of disease-TCM.
Time frame: 3 years
Concentration of GCSF
Concentration of GCSF will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of GCSF in the cell models of disease-TCM syndrome
Concentration of GCSF will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of GM-CSF
Concentration of GM-CSF will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of GM-CSF in the cell models of disease-TCM syndrome
Concentration of GM-CSF will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of I-309 (TCA-3/CCL1)
Concentration of I-309 (TCA-3/CCL1) will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of I-309 (TCA-3/CCL1) in the cell models of disease-TCM syndrome
Concentration of I-309 (TCA-3/CCL1) will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of ICAM-1(CD54)
Concentration of ICAM-1(CD54) will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of ICAM-1(CD54) in the cell models of disease-TCM syndrome
Concentration of ICAM-1(CD54) will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of IFN-gamma
Concentration of IFN-gamma will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of IFN-gamma in the cell models of disease-TCM syndrome
Concentration of IFN-gamma will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of IL-1 alpha (IL-1 F1)
Concentration of IL-1 alpha (IL-1 F1) will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of IL-1 alpha (IL-1 F1) in the cell models of disease-TCM syndrome
Concentration of IL-1 alpha (IL-1 F1) will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of IL-1 beta (IL-1 F2)
Concentration of IL-1 beta (IL-1 F2) will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of IL-1 beta (IL-1 F2) in the cell models of disease-TCM syndrome
Concentration of IL-1 beta (IL-1 F2) will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of IL-1 ra (IL-1 F3)
Concentration of IL-1 ra (IL-1 F3) will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of IL-1 ra (IL-1 F3) in the cell models of disease-TCM syndrome
Concentration of IL-1 ra (IL-1 F3) will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of IL-2
Concentration of IL-2 will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of IL-2 in the cell models of disease-TCM syndrome
Concentration of IL-2 will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of IL-4
Concentration of IL-4 will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of IL-4 in the cell models of disease-TCM syndrome
Concentration of IL-4 will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of IL-5
Concentration of IL-5 will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of IL-5 in the cell models of disease-TCM syndrome
Concentration of IL-5 will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of IL-6
Concentration of IL-6 will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of IL-6 in the cell models of disease-TCM syndrome
Concentration of IL-6 will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of IL-6R
Concentration of IL-6R will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of IL-6R in the cell models of disease-TCM syndrome
Concentration of IL-6R will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of IL-7
Concentration of IL-7 will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of IL-7 in the cell models of disease-TCM syndrome
Concentration of IL-7 will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of IL-8(CXCL8)
Concentration of IL-8(CXCL8) will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of IL-8(CXCL8) in the cell models of disease-TCM syndrome
Concentration of IL-8(CXCL8) will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of IL-10
Concentration of IL-10 will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of IL-10 in the cell models of disease-TCM syndrome
Concentration of IL-10 will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of IL-11
Concentration of IL-11 will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of IL-11 in the cell models of disease-TCM syndrome
Concentration of IL-11 will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of IL-12 p40
Concentration of IL-12 p40 will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of IL-12 p40 in the cell models of disease-TCM syndrome
Concentration of IL-12 p40 will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of IL-12 p70
Concentration of IL-12 p70 will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of IL-12 p70 in the cell models of disease-TCM syndrome
Concentration of IL-12 p70 will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of IL-13
Concentration of IL-13 will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of IL-13 in the cell models of disease-TCM syndrome
Concentration of IL-13 will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of IL-15
Concentration of IL-15 will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of IL-15 in the cell models of disease-TCM syndrome
Concentration of IL-15 will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of IL-16
Concentration of IL-16 will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of IL-16 in the cell models of disease-TCM syndrome
Concentration of IL-16 will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of IL-17A
Concentration of IL-17A will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of IL-17A in the cell models of disease-TCM syndrome
Concentration of IL-17A will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of MCP-1 (CCL2)
Concentration of MCP-1 (CCL2) will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of MCP-1 (CCL2) in the cell models of disease-TCM syndrome
Concentration of MCP-1 (CCL2) will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of M-CSF
Concentration of M-CSF will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of M-CSF in the cell models of disease-TCM syndrome
Concentration of M-CSF will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of MIG (CXCL9)
Concentration of MIG (CXCL9) will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of MIG (CXCL9) in the cell models of disease-TCM syndrome
Concentration of MIG (CXCL9) will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of MIP-1
Concentration of MIP-1 will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of MIP-1 in the cell models of disease-TCM syndrome
Concentration of MIP-1 will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of alpha (CCL3) in the cell models of disease-TCM syndrome
Concentration of alpha (CCL3) will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of alpha (CCL3)
Concentration of alpha (CCL3) will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of MIP-1 beta (CCL4)
Concentration of MIP-1 beta (CCL4) will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of MIP-1 beta (CCL4) in the cell models of disease-TCM syndrome
Concentration of MIP-1 beta (CCL4) will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of MIP-1 delta (CCL15)
Concentration of MIP-1 delta (CCL15) will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of MIP-1 delta (CCL15) in the cell models of disease-TCM syndrome
Concentration of MIP-1 delta (CCL15) will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of PDGF-BB
Concentration of PDGF-BB will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of PDGF-BB in the cell models of disease-TCM syndrome
Concentration of PDGF-BB will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of RANTES (CCL5)
Concentration of RANTES (CCL5) will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of RANTES (CCL5) in the cell models of disease-TCM syndrome
Concentration of RANTES (CCL5) will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of TIMP-1
Concentration of TIMP-1 will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of TIMP-1 in the cell models of disease-TCM syndrome
Concentration of TIMP-1 will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of TIMP-2
Concentration of TIMP-2 will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of TIMP-2 in the cell models of disease-TCM syndrome
Concentration of TIMP-2 will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of TNF alpha
Concentration of TNF alpha will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of TNF alpha in the cell models of disease-TCM syndrome
Concentration of TNF alpha will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of TNF beta (TNFSF1B)
Concentration of TNF beta (TNFSF1B) will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of TNF beta (TNFSF1B) in the cell models of disease-TCM syndrome
Concentration of TNF beta (TNFSF1B) will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of TNF RI (TNFRSF1A)
Concentration of TNF RI (TNFRSF1A) will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of TNF RI (TNFRSF1A) in the cell models of disease-TCM syndrome
Concentration of TNF RI (TNFRSF1A) will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of TNF RII(TNFRSF1B)
Concentration of TNF RII(TNFRSF1B) will be detected by ELISA in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of TNF RII(TNFRSF1B) in the cell models of disease-TCM syndrome
Concentration of TNF RII(TNFRSF1B) will be detected by ELISA in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of Signaling pathway indicator
Concentration of Signaling pathway indicator will be detected by ELISA, metabonomics, proteomics, transcriptomics in LKD group, PBS group and NC group.
Time frame: 3 years
Concentration of Signaling pathway indicator in the cell models of disease-TCM syndrome
Concentration of Signaling pathway indicator will be detected by ELISA, metabonomics, proteomics, transcriptomics in the cell models of disease-TCM syndrome.
Time frame: 3 years
Concentration of Routine Blood Examination （Concentration of WBC, LYMPH%, MONO, NEUT%, LYMPH, MONO%, NEUT, BASO%, BASO, MCH, MCHC,ROW-CV, MPV, PLT, PDW, EO%, P-LCR, RBC, Hb, HCT）
LKD group, PBS group and NC group's Routine Blood Examination will be detect by automated hematology analyzer.
Time frame: 3 years
Concentration of Blood Biochemistry (Concentration of TC, TG, HDL-C, LDL-C, Urea, Cr, UA, GLu, ALT, AST, TP, ALB, ALP, GGT, TBIL, DBIL, Mg, K, Na, Cl, Ca, P, Fe, CO2)
LKD group, PBS group and NC group's Blood Biochemistry will be detect by automatic biochemical analyzer.
Time frame: 3 years
Concentration of Routine Urine Examination (Concentration of LEU, NIT, PRO, GLU-U, KET, URO, BIL, PH-U, SG, BLU)
LKD group, PBS group and NC group's Routine Urine Examination will be detect by Urine analyzer.
Time frame: 3 years
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