This study aims to detect the presence and diversity of Tissue resident memory T cell populations in early and late cases of generalized non-segmental Vitiligo.
* Written informed consent obtained from every participant. * Detailed history taking including onset, course, duration of the disease, aggravating factors and received medications * VASI score assessed in every patient. * Patients photographed, divided into two groups early \& late cases and 3 mm punch biopsies will be taken from perilesional and non lesional skin. * Biopsies processed and stained for CD8 \& CD69 in cases of early group, \& late group and controls by immunofluorescence.
Study Type
OBSERVATIONAL
Enrollment
29
Kasr El Ainy University hospital
Cairo, Egypt
CD8+ immunofluorescence
Number of Tissue resident Memory cells by positively stained by CD8+ in early and late generalized non segmental vitiligo patients
Time frame: 6months-1 year
CD69+ immunofluorescence
Number of Tissue resident Memory cells by positively stained by CD8+ in early and late generalized non segmental vitiligo patients
Time frame: 6months-1 year
Double CD8+ and CD69+ immunofluorescence
Number of Tissue resident Memory cells by double stained by CD8+ \&CD 69+ in early and late generalized non segmental vitiligo patients
Time frame: 6months-1 year
VASI
Vitiligo Area and Severity index minimum 0 maximum 100, the larger the number the worse the condition
Time frame: 6months-1 year
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