Macrophage Markers in Periodontal and Peri-implant Health and Disease

Biruni University36 enrolled

Overview

Macrophage surface markers (CD80, CD163 and CD206) will be evaluated in periodontally healthy gingiva, healthy peri-implant mucosa, and periodontitis and peri-implantitis lesions.

Soft tissue biopsies obtained from clinically healthy gingiva, healthy peri-implant mucosa, Stage III Grade C periodontitis lesions and peri-implantitis lesions will be evaluated. Healthy peri-implant samples are obtained from submerged implants during exposure surgery. Healthy gingiva samples are obtained from individuals receiving crown lengthening, gingivectomy or tooth extraction as excess tissue during the corresponding treatment. Samples from peri-implantitis and periodontitis (pocket depth ≥6 mm, bleeding on probing+) are obtained during initial periodontal treatment / debridement procedure with a single stroke at the pocket wall. The samples will be grinded and ultrasonicated. Western blotting will be used for determination of CD80, CD163 and CD206 in biopsy specimens. ImageJ will be used for intensity analysis of the bands.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Peri-Implantitis Periodontitis

Eligibility

Sex: ALLMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * diagnosed with generalized Grade C Stage III periodontitis * diagnosed with peri-implantitis * periodontally healthy individuals with dental implants in the primary healing period * periodontally healthy individuals who are in need of crown lengthening / gingivectomy or extraction Exclusion Criteria: * systemic disease * smoking / former smokers * regular medicine intake * antibiotic or anti-inflammatory medicine intake in the last 3 months * pregnancy or lactation

Locations (1)

Biruni University

Istanbul, Turkey (Türkiye)

Outcomes

Primary Outcomes

CD80

Tissue levels of CD80 Western Blot assay analyzed using Image J software: Following scanning for optical densitometry, the gels were analyzed with Image J software by obtaining profile plots, drawing lines enclosing the interest area and measuring the peak area of interest with the wand tool. The calculated optical density values are presented as arbitrary density units.

Time frame: Baseline

CD163

Tissue levels of CD163 Western Blot assay analyzed using Image J software: Following scanning for optical densitometry, the gels were analyzed with Image J software by obtaining profile plots, drawing lines enclosing the interest area and measuring the peak area of interest with the wand tool. The calculated optical density values are presented as arbitrary density units.

Time frame: Baseline

CD206

Tissue levels of CD206 Western Blot assay analyzed using Image J software (arbitrary density units): Following scanning for optical densitometry, the gels were analyzed with Image J software by obtaining profile plots, drawing lines enclosing the interest area and measuring the peak area of interest with the wand tool. The calculated optical density values are presented as arbitrary density units.

Time frame: Baseline

CD80/CD163 Ratio

Tissue levels (Western Blot assay analyzed using Image J software) of CD80 divided by those of CD163

Time frame: Baseline

CD80/CD206 Ratio

Tissue levels (Western Blot assay analyzed using Image J software) of CD80 divided by those of CD206

Time frame: Baseline

Secondary Outcomes

Probing Depth

Probing depth of each site, where the tissue sample was harvested. The average probing depth of sites in each group are presented.

Data from ClinicalTrials.gov

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