Characterization of Bovine Adrenal Medulla as a New Surrogate Model of Non-histaminergic Itch

Aalborg University22 enrolled

Overview

With this experiment, the experimenter wish evaluate the role of TRPA1 on non-histaminergic itch induced by BAM8-22

With this experiment, the experimenter wish evaluate the role of TRPA1 on non-histaminergic itch induced by BAM8-22 2 (Bovine Adrenal Medulla), which is found naturally in the human body and which has proved to evoke itch. To evaluate the role of TRPA1 it will be used a TRPA1 antagonist (A-967079)

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

SINGLE

Enrollment

Conditions

Itch

Interventions

A-967079 and BAM8-22 applicationDRUG

A-967079 solution (in PBS 0.5 mg/ml, subcutaneous injection of 0.1ml) and BAM8-22 with inactivate cowhage spicules 20 µl of BAM8-22 will be applied to a previously determined area on the volar forearm using inactivate cowhage spicules

A-967079 and BAM8-22 application after 5 minutes.DRUG

A-967079 solution (in PBS 0.5 mg/ml, subcutaneous injection of 0.1ml). BAM8-22 with inactivate cowhage spicules 20 µl of BAM8-22 will be applied 5 minutes after to a previously determined area on the volar forearm using inactivate cowhage spicules

A-967079 application.DRUG

A-967079 solution (in PBS 0.5 mg/ml, subcutaneous injection of 0.1ml) will be applied to a previously determined area on the volar forearm

Eligibility

Sex: ALLMin age: 18 YearsMax age: 60 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Healthy men and women * 18-60 years * Speak and understand English Exclusion Criteria: * Pregnancy or lactation * Drug addiction defined as any use of cannabis, opioids or other drugs * Previous or current history of neurological, dermatological, immunological musculoskeletal, cardiac disorder or mental illnesses that may affect the results (e.g. neuropathy, muscular pain in the upper extremities, etc.) * Moles, wounds, scars or tattoos in the area to be treated or tested * Lack of ability to cooperate • * Current use of medications that may affect the trial such as antihistamines and pain killers. * Skin diseases * Consumption of alcohol or painkillers 24 hours before the study days and between these - Acute or chronic pain • * Participation in other trials within 1 week of study entry (4 weeks in the case of pharmaceutical trials)

Locations (1)

Giulia Erica Aliotta

Aalborg, Denmark