Microchimeric cells are known to be transferred from mother to child through the placenta. However it has been postulated that they could also be transferred through breastfeeding. This study is designed to analyse lymphoid and digestive tract tissues (tonsils and appendixes) in male infants (or young adults) to research microchimeric female cells and try to correlate the frequency and localisation of said cells to the breastfeeding status during early infancy
Study Type
OBSERVATIONAL
Enrollment
80
Uh Montpellier
Montpellier, France
RECRUITINGTo identify microchimeric cells of maternal origin in lymphoid
To identify microchimeric cells of maternal origin in lymphoid in young males cytogenetic FISH staining, coupled with MS-PCR, to identify microchimeric cells.
Time frame: day 1
To identify microchimeric cells of maternal origin in digestive tissues
To identify microchimeric cells of maternal origin in digestive tissues in young males cytogenetic FISH staining, coupled with MS-PCR, to identify microchimeric cells.
Time frame: day 1
to determine Quantifying the microchimeric cells
Quantifying the microchimeric cells : Cell quantification will be assessed through FISH staining (automated cell counting) and qPCR
Time frame: day 1
to determine locating the cells in tissues
locating the cells in tissues : Appendix histology slides will be analysed after FISH staining to locate the microchimeric cells
Time frame: day 1
to determine trying to find a correlation between breastfeeding status
trying to find a correlation between breastfeeding status : Microchimeric cell quantification will be confronted to the breastfeeding status in infancy (collected during patient inclusion) to validate a correlation
Time frame: day 1
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