The Acute Effects of Meals Rich in Saturated or Unsaturated Fatty Acids on Postprandial Lipaemia in Healthy Men

University of Reading13 enrolled

Overview

Current dietary recommendations suggest that lowering intake of saturated fats or replacing it with unsaturated fats will decrease the risk of developing cardiovascular disease. Coconut oil has gained popularity in recent years but it contains 90% saturated fat, which has higher percentage of saturated fat than butter. To date, only limited studies have determined the acute effects of meals containing coconut oil on blood lipids, but findings are inconsistent. Therefore, further studies are needed to address this knowledge gap and compare the postprandial effects of test meals rich in coconut oil with other sources of saturated fatty acids such as butter and unsaturated fatty acids (vegetable oils). A cross-over, double-blind, randomised acute postprandial study will be conducted in 15 healthy men. Participants will be assigned to consume the test meals rich in saturated or unsaturated fatty acids in random order on 3 separate occasions, with 3-4 weeks between each study visit. Participants will be provided with breakfast (toast with jam and milkshake, 50g fat) and lunch (toast with jam and milkshake, 30g fat). The anthropometric, blood pressure, arterial stiffness, and breath samples will be taken for each study visit. Blood samples will be collected for the measurement of fasting lipids, glucose, insulin, inflammatory markers, whole blood culture as well as blood clotting. Breath samples are collected for the measurement of gastric emptying as well as assessment of satiety using questionnaires (100 mm visual analogue scale) completed throughout the day. The findings from this study will contribute to the evidence base on how consuming meals rich in coconut oil influence the level of blood lipids as well as other biomarkers for cardiovascular disease.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

TREATMENT

Masking

SINGLE

Enrollment

Eligibility

Sex: MALEMin age: 30 YearsMax age: 70 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Healthy men * Aged between 30 to 70 years * Serum triacylglycerol \< 2.3 mmol/l * Body mass index between 19-32 kg/m2 * Total cholesterol \< 7.5 mmol/l Exclusion Criteria: * Females * Smokers * Medical history of myocardial infarction or stroke in the past 12 months * Diabetes (defined as fasting glucose \> 7.0 mmol/l) or other endocrine disorders * Kidney, liver, pancreas or gastrointestinal disorder * Hypertension (blood pressure \> 140/90 mmHg), cancer, medication for hyperlipidaemia (e.g. statins), hypertension or, inflammation * Anaemia (\<130 g/L haemoglobin) * Taking any dietary supplements known to influence lipids/gut microbiota (e.g. plant stanols, fish oil, phytochemicals, natural laxatives, probiotics and prebiotics) * Drinking in excess of 14 units of alcohol per week * Planning on a weight-reducing regime * Parallel participation in another dietary intervention study * Any other unusual medical history or diet and lifestyle habits or practices that would preclude volunteers from participating in a dietary intervention and metabolic study

Outcomes

Primary Outcomes

Change from baseline in postprandial triacylglycerol

Triacylglycerol

Time frame: Acute study: taken at 0 (baseline), 30, 60, 90, 120, 180, 240, 300, 330, 360, 390, 420, 480 minutes

Secondary Outcomes

Fasting blood lipids

Total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol

Time frame: Acute study: taken at 0 (baseline)

Change from baseline in postprandial non-esterified fatty acids

Non-esterified fatty acids

Time frame: Acute study: taken at 0 (baseline), 30, 60, 90, 120, 180, 240, 300, 330, 360, 390, 420, 480 minutes

Change from baseline in postprandial insulin

Insulin

Time frame: Acute study: taken at 0 (baseline), 30, 60, 90, 120, 180, 240, 300, 330, 360, 390, 420, 480 minutes

Change from baseline in postprandial vascular stiffness

Measured via pulse wave assessment using the Mobil-O-graph device

Time frame: Acute study: taken at 0 (baseline), 150, 315, 465 minutes

Change from baseline in postprandial blood pressure

Systolic and diastolic blood pressure

Time frame: Acute study: taken at 0 (baseline), 180, 300, 480 minutes

Change from baseline in postprandial thrombin generation

Thrombin generation

Time frame: Acute study: taken at 0 (baseline), 180, 330, 480 minutes

Change from baseline in postprandial gastric emptying

Assessed using gas chromatography-mass spectrometry

Time frame: Acute study: taken at 15 minutes intervals between 0 min (baseline) and 480 minutes

Appetite rating

Assessed using 100 mm visual analogue questionnaire

Time frame: Acute study: taken at 30 minutes intervals after the test meals up to 8 hours

Change from baseline in postprandial gut hormones

Ghrelin, peptide YY, gastric inhibitory polypeptide, glucagon-like peptide-1

Time frame: Acute study: taken at 0 (baseline), 30, 60, 120, 240, 330, 360, 390 & 480 minutes

Change from baseline in postprandial endothelial activation

E-selectin, P-selectin, vascular cell adhesion molecule 1, intercellular adhesion molecule-1

Time frame: Acute study: taken at 0 (baseline),180, 300 and 420 minutes

Change from baseline in postprandial cytokine

Interleukin 6, interleukin 1 beta, interleukin 10, tumour necrosis factor alpha, C-C motif chemokine ligand 5, C-X-C motif chemokine ligand 5

Time frame: Acute study: taken at 0 (baseline),180, 300 and 420 minutes

Weight

Body mass index will be calculated (kg/ height in m\^2)

Time frame: Acute study: taken at 0 (baseline)

Body composition

Fat mass, fat free mass, trunk fat, trunk fat mass, trunk fat free mass

Time frame: Acute study: taken at 0 (baseline)

Habitual diet assessment

4-day food diary

Time frame: Prior to the start of the study

The Acute Effects of Meals Rich in Saturated or Unsaturated Fatty Acids on Postprandial Lipaemia in Healthy Men

Overview

Conditions

Interventions

Eligibility

Locations (1)

Outcomes

Primary Outcomes

Secondary Outcomes