The purpose of this study is to cast light on the highly complex etiology and cellular landscape of hip osteoarthritis by utilising single-cell and spatial omics.
The specific objectives of this project are: 1. Using the latest single-cell RNA sequencing (scRNAseq) techniques the investigators aim to A) characterize what kind of cell populations are found in different synovial tissues and blood derived samples of OA patients, B) determine how the cell composition differs between arthritic and corresponding non-arthritic tissues, C) map the transcriptional and regulatory landscape of the cells mentioned in A and B focusing on the inflammatory responses, D) determine what are the key molecular pathways activated in OA. 2. To determine if some of the blood-derived immune cell populations or their products could be used as biomarkers for OA. 3. To map the whole transcriptome and proteome of OA and non-arthritic control tissue while keeping the morphological context with spatial omics technologies. 4. Further differentiation and identification of OA endotypes utilizing the single-cell and spatial omics data. The project includes a Rheumatoid sub-study where the main objective is to compare arthritic tissue and peripheral blood constituents between OA and rheumatoid arthritis patients to explore the differences in the disease mechanisms.
Study Type
OBSERVATIONAL
Enrollment
110
Hip joint replacement surgery. Elective for RA and OA cases.
PET-centre, University of Turku
Turku, Southwest Finland, Finland
ACTIVE_NOT_RECRUITINGTurku Bioscience, University of Turku
Turku, Southwest Finland, Finland
ACTIVE_NOT_RECRUITINGTurku University Hospital
Turku, Southwest Finland, Finland
RECRUITINGCharacterization of cell populations in OA
Characterization of cell populations found in different synovial tissues and blood derived samples of OA patients utilising single-cell RNA sequencing solutions.
Time frame: Starting during the first quarter of 2025, ending by the last quarter of 2026.
Comparison of cell populations between OA cases and controls
The investigators will determine how the cell composition differs between arthritic and corresponding non-arthritic tissues utilising single-cell RNA sequencing solutions.
Time frame: Starting during the first quarter of 2025, ending by the last quarter of 2026.
Cellular landscape in OA
The investigators will map the transcriptional, regulatory and protein landscape of OA at single-cell and tissue (spatial) level.
Time frame: Starting during the last quarter of 2024, ending by the last quarter of 2026.
Key molecular pathways of OA
The investigators will determine what are the key molecular pathways activated in OA.
Time frame: Starting during the last quarter of 2025, ending by the last quarter of 2027.
Comparison of disease mechanisms between RA and OA
In the Rheumatoid sub-study the investigators will explore the differences in the disease mechanisms between OA and RA by comparing synovial tissues and peripheral blood sample constituents.
Time frame: Starting during the last quarter of 2024, ending by the last quarter of 2028.
Biomarkers for OA
The investigators will investigate if some of the blood-derived immune cell populations or their products could be used as biomarkers for OA.
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Helsinki University Hospital
Espoo, Uusimaa, Finland
RECRUITINGTime frame: Starting during the second half of 2026, ending by the last quarter of 2028.
OA endotypes
The investigators aim to identify and further differentiate OA endotypes by utilizing the single-cell and spatial data.
Time frame: Starting during the first half of 2025, ending by the second half of 2027.