The aim of this study is to assess the levels of galectin-3 in the GCF and saliva in patient with gingivitis and patient with stage III periodontitis.
The aim of this study is to assess the levels of galectin-3 in the GCF and saliva in patient with gingivitis and patient with stage III periodontitis. This study will be conducted in the Oral Medicine and Periodontology department, Faculty of Dentistry- Cairo University, Egypt. Patients will be selected from the outpatient clinic of the department of Oral Medicine and Periodontology, Faculty of Dentistry-Cairo University.
Study Type
OBSERVATIONAL
Enrollment
66
GCF sample collection by Enzyme-Linked Immunosorbent Assay (ELISA)
Saliva sample collection by Enzyme-Linked Immunosorbent Assay (ELISA)
Cairo university Faculty of dentistry
Cairo, Egypt
galectin-3 in GCF
The total amount of Galectin-3 in GCF
Time frame: 24 hours after taking the clinical measurements at the first visit
galectin-3 in saliva.
The total amount of Galectin-3 in saliva
Time frame: 24 hours after taking the clinical measurements at the first visit
Pocket depth
in mm
Time frame: 24 hours before taking GCF and salivary samples
Clinical attachment level
in mm
Time frame: 24 hours before taking GCF and salivary samples
Bleeding on Probing
By gently probing the orifice of the gingival crevice, the periodontal probe will be inserted 1 to2 mm into the gingival sulcus starting at one interproximal area and moving to the other. If bleeding occurs within 10 seconds a positive finding is recorded.
Time frame: 24 hours before taking GCF and salivary samples
Plaque Index
Scores: 0: No plaque 1. A film of plaque adhering to the free gingival margin and adjacent area of the tooth. The plaque may be seen insitu only after application of a disclosing solution or by using the probe on the tooth surface. 2. Moderate accumulation of soft deposit s within the gingival pocket, or the tooth and gingival margin, which can be seen with the naked eye. 3. Abundance of soft matter within the gingival pocket and/or on the tooth and gingival margin.
Time frame: 24 hours before taking GCF and salivary samples
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