E-cadherin and Integrin Alpha v Beta 1 in Vitiligo

Kasr El Aini Hospital15 enrolled

Overview

The purpose of this clinical trial is to study the effect of Narrow Band UVB on E-cadherin and integrin alpha v beta 1 as representatives of adhesion molecules in non-segmental vitiligo in a trial to verify a novel proposed mechanism of Narrow Band UVB. Additionally, specific melanocyte marker (Melan A) will be done to settle the localization of adhesion molecules in relation to melanocytes.

Study Type

INTERVENTIONAL

Allocation

Purpose

TREATMENT

Masking

NONE

Enrollment

Conditions

Vitiligo

Interventions

Narrow band UVBDEVICE

Narrow band UVB emitting UVB at wavelength 311nm

Eligibility

Sex: ALLMin age: 18 YearsMax age: 60 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * All clinically diagnosed cases of non-segmental vitiligo . * Patients with VIDA +1 or more (new/expanding lesions active in time period of 6-12 months ago) will be included in the trial. Activity of vitiligo will be evaluated through Vitiligo Disease Activity (VIDA) Score. Exclusion Criteria: * Patients who have stable disease at the time of study i.e. no progression of existing disease or appearance of new lesions in the last six months. * Patients receiving treatment for vitiligo 3 months prior to the study. * Patients with contraindications for phototherapy. * Patients with current/history of any systemic and/or dermatological diseases affecting the immune system as autoimmune diseases and malignancy.

Locations (1)

Kasr El Ainy university hospital, Faulty of medicine,Cairo university

Cairo, El Manial, Egypt

Outcomes

Primary Outcomes

Melanocyte marker (Melan A) for staining of melanocytes

Immunohistochemistry and image analysis Data were obtained using Leica Quinn software with Olympus BX40 microscope and Panasonic camera Measurements were taken in 5 non-overlapping high power fields.

Time frame: 4 months

E-cadherin expression as a cell adhesion molecule between melanocytes and keratinocytes

Immunohistochemistry and image analysis Data were obtained using Leica Quinn software with Olympus BX40 microscope and Panasonic camera Measurements were taken in 5 non-overlapping high power fields.

Time frame: 4 months

Integrin alpha v beta 1 expression as a cell adhesion molecule between melanocytes and keratinocytes

Immunohistochemistry and image analysis Data were obtained using Leica Quinn software with Olympus BX40 microscope and Panasonic camera Measurements were taken in 5 non-overlapping high power fields.

Time frame: 4 months

Secondary Outcomes

Vitiligo Area and severity index (VASI)

Minimum value is 0, maximum value is 100 Higher scores mean worse outcome

Time frame: 4 months

Data from ClinicalTrials.gov

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