A Dose Escalation Study to Evaluate the Effect of RT234 in Subjects With Pulmonary Arterial Hypertension

Inclusion Criteria: 1. Between 18 and 80 years of age, inclusive. 2. Diagnosis of RHC-confirmed WHO Group 1 PAH in any of the following three categories: Idiopathic, primary or familial pulmonary arterial hypertension (IPAH, PPH, or FPAH); OR PAH associated with one of the following connective tissue diseases (CTD): 1. Systemic sclerosis (scleroderma) 2. Limited scleroderma 3. Mixed connective tissue disease 4. Systemic lupus erythematosus 5. Overlap syndrome 6. Other autoimmune disorders; OR PAH associated with: 1. Human immunodeficiency virus (HIV) infection with no evidence of opportunistic infection in the preceding 6 months; 2. Simple, congenital systemic-to-pulmonary shunts at least one-year post-surgical repair. 3. Exposure to legal drugs, chemicals and toxins, such as fenfluramine, derivatives, other anorexigens, toxic rapeseed oil or L-tryptophan. Subjects with PAH associated with illegal drug use, such as methamphetamine, were excluded. 3. Previous diagnosis with PAH with the following conditions: 1. Stable PAH without significant adjustments of disease-specific background PAH therapy, at least 3 months prior to RHC procedure; 2. If on corticosteroids, has been receiving a stable dose of ≤ 20 mg/day of prednisone (or equivalent dose of other corticosteroid) for at least 30 days prior to RHC procedure. 4. Pulmonary Function Tests within 24 months prior to RHC procedure that fulfilled the following criteria (pulmonary function; (PFT may be assessed at Screening if historical PFT results are not available): 1. Forced Expiratory volume in one second (FEV1) ≥ 60% predicted (pre-bronchodilators); 2. FEV1/ forced expiratory vital capacity (FVC) ≥ 60% (pre-bronchodilators); 3. FVC ≥ 60% predicted. Exclusion Criteria: 1. Baseline systemic hypotension, defined as MAP \< 50 mmHg or systolic blood pressure (SBP)\< 90 mmHg at Screening. 2. Requirement of intravenous inotropes within 30 days prior to RHC procedure. 3. Use of oral, topical or inhaled nitrates within 14 days prior to RHC procedure. 4. Uncontrolled systemic hypertension: SBP \> 160 mmHg or diastolic blood pressure (DBP) \>100 mmHg at Screening. 5. History of portal hypertension or chronic liver disease, including active viral replication of hepatitis B and/or hepatitis C or classified as having moderate to severe hepatic impairment (Child-Pugh Class B-C). 6. Chronic renal insufficiency as defined by serum creatinine \> 2.5 mg/dL at Screening or requires dialysis. 7. History of atrial septostomy. 8. Unrepaired congenital heart disease (CHD). 9. Pericardial constriction; restrictive or congestive cardiomyopathy. 10. History of left ventricular ejection fraction (EF) \< 40% by multiple gated acquisition scan (MUGA), angiography, echocardiography, or cardiac magnetic resonance imaging (CMRI). 11. Symptomatic coronary disease with demonstrable ischemia. 12. Poorly controlled asthma defined by active wheezing and/or cough at the time of Screening or day of participation in Parts A and B. 13. Clinically significant intercurrent illness (including lower respiratory tract infection) or clinically significant surgery within 30 days prior to study drug administration. 14. Clinical RHC \< 14 days prior to Screening. 15. History of non-arteritic anterior ischemic optic neuropathy (NAION) or retinitis pigmentosa.