The process of removing bacteria and their products from the root canals is an essential step that is achieved through the combination of mechanical preparation and irrigation with chemicals. However, mechanical preparation alone can not reduce the microbial formations inside root canals, so at least about 35% of the root canal walls remain without the preparation tools reaching them. Many irrigants were used to irrigate the root canals, as sodium hypochlorite and chlorhexidine are the most famous. Although most studies have proven the effectiveness of sodium hypochlorite with its different concentrations in accomplishing this task, some of them showed the inability of the irrigant fluid to eliminate Enterococcus faecalis inside the canals. These bacteria are highly resistant, and therefore endodontic treatment fails in the long term.
Bacteria and their products play an essential role in the initiation and persistence of endodontic diseases. Therefore, eliminating them and preventing the return of their effectiveness is the desired goal in any successful endodontic treatment in the short and long term. Due to the fact that mechanical preparation of the root canal alone is not capable of removing the entire bacterial content, chemical irrigants were considered necessary to reduce the number of bacteria and toxins resulting from them. Until now, there is no irrigant capable of removing the entire bacterial content from the root canal. Despite the many advantages of sodium hypochlorite, several studies have revealed the toxicity of the solution in high concentrations of it, and it tends to cause tissue irritation when it comes into contact with the apical tissues. Chlorhexidine has been used for a long time in dentistry due to its antibacterial properties, long period of effect, and relatively low toxicity. This has prompted its use as an irrigant and an intra-canal dressing in endodontic treatment.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
TRIPLE
Enrollment
15
* Irrigate the root canal with 1 ml of 2% CHx solution between each file 1 mm from the apical foramen by using a 27-gauge irrigation needle. * Irrigate the root canal with 3 ml of the solution after using the last file. * Irrigation with 3 ml of 0.5% Tween 80 and 0.07 % lecithin to remove residual chlorhexidine solution. * Drying the canal with paper points (placing a 20 size paper point for 60 seconds in each canal) to take the bacterial smear. * Follow-up of endodontic treatment procedures for the treated tooth.
* Irrigate the root canal with 1 ml of 2% CHx solution between each file 1 mm from the apical foramen by using a 27-gauge irrigation needle with activation using an ultrasonic irrigation head. * Irrigate the root canal with 3 ml of the solution after using the last file with activation using an ultrasonic irrigation head. * Irrigation with 3 ml of 0.5% Tween 80 and 0.07 % lecithin to remove residual chlorhexidine solution. * Drying the canal with paper points (placing a 20 size paper point for 60 seconds in each canal) to take the bacterial smear. * Follow-up of endodontic treatment procedures for the treated tooth.
* Irrigate the root canal with 1 ml of 2% CHx gel between each file 1 mm from the apical foramen by using a 27-gauge irrigation needle. * Irrigate the root canal with 3 ml of the gel after using the last file. * Irrigation with 3 ml of 0.5% Tween 80 and 0.07 % lecithin to remove residual chlorhexidine gel. * Drying the canal with paper points (placing a 20 size paper point for 60 seconds in each canal) to take the bacterial smear. * Follow-up of endodontic treatment procedures for the treated tooth.
* Irrigate the root canal with 1 ml of 2% CHx gel between each file 1 mm from the apical foramen by using a 27-gauge irrigation needle with activation using an ultrasonic irrigation head. * Irrigate the root canal with 3 ml of the gel after using the last file with activation using an ultrasonic irrigation head. * Irrigation with 3 ml of 0.5% Tween 80 and 0.07 % lecithin to remove residual chlorhexidine gel. * Drying the canal with paper points (placing a 20 size paper point for 60 seconds in each canal) to take the bacterial smear. * Follow-up of endodontic treatment procedures for the treated tooth.
* Irrigate the root canal with 1 ml of 5.25% sodium hypochlorite solution between each file 1 mm from the apical foramen by using a 27-gauge irrigation needle. * Irrigate the root canal with 3 ml of the solution after using the last file. * Irrigating the canal with 3 ml of 5% sodium thiosulfate solution to remove residual of sodium hypochlorite. * Drying the canal with paper points (placing a 20 size paper point for 60 seconds in each canal) to take the bacterial smear. * Follow-up of endodontic treatment procedures for the treated tooth.
Damascus University
Damascus, Syria
Logarithmic reduction of bacterial count within the root canal of chlorhexidine solution.
Set the Petri dish on a grid background and count the colonies in each grid cell, moving in a methodical pattern through all of the cells.
Time frame: 24 hours after sample incubation
Logarithmic reduction of bacterial count within the root canal of activated chlorhexidine solution.
Set the Petri dish on a grid background and count the colonies in each grid cell, moving in a methodical pattern through all of the cells.
Time frame: 24 hours after sample incubation
Logarithmic reduction of bacterial count within the root canal of chlorhexidine gel.
Set the Petri dish on a grid background and count the colonies in each grid cell, moving in a methodical pattern through all of the cells.
Time frame: 24 hours after sample incubation
Logarithmic reduction of bacterial count within the root canal of activated chlorhexidine gel.
Set the Petri dish on a grid background and count the colonies in each grid cell, moving in a methodical pattern through all of the cells.
Time frame: 24 hours after sample incubation
Logarithmic reduction of bacterial count within the root canal of sodium hypochlorite solution.
Set the Petri dish on a grid background and count the colonies in each grid cell, moving in a methodical pattern through all of the cells.
Time frame: 24 hours after sample incubation
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.