The study will assess the exercise-induced changes in chromatin accessibility and gene expression in a cell type-specific manner in skeletal muscle from healthy adults in response to exercise at three different intensities - 1) low-intensity continuous exercise, 2) moderate-intensity continuous exercise, and 3) high-intensity interval exercise.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
45
subjects will exercise at 30% maximal oxygen consumption (VO2max) for 45-60 minutes
subjects will exercise at 65% maximal oxygen consumption (VO2max) for 30-45 minutes
subjects will completed 10x1minute exercise intervals at maximal oxygen consumption (VO2max) with 1 minute active recovery between
University of Michigan
Ann Arbor, Michigan, United States
post-translational modifications of skeletal muscle oxidative enzymes
Western blot
Time frame: 30 minutes before exercise
post-translational modifications of skeletal muscle oxidative enzymes
Western blot
Time frame: immediately after exercise
post-translational modifications of skeletal muscle oxidative enzymes
Western blot
Time frame: 60 minutes after exercise
post-translational modifications of Adipose tissue metabolic proteins
Western blot
Time frame: 15 minutes before exercise
post-translational modifications of Adipose tissue metabolic proteins
Western blot
Time frame: 75 minutes after exercise
mRNA expression of skeletal muscle oxidative enzymes
qPCR
Time frame: 30 minutes before exercise
mRNA expression of skeletal muscle oxidative enzymes
qPCR
Time frame: immediately after exercise
mRNA expression of skeletal muscle oxidative enzymes
qPCR
Time frame: 60 minutes after exercise
mRNA expression of adipose tissue metabolic enzymes
qPCR
Time frame: 15 minutes before exercise
mRNA expression of adipose tissue metabolic enzymes
qPCR
Time frame: 75 minutes after exercise
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