Evaluation of technical feasibility for Homologous Recombination (HR) genes variants research on circulating tumor DNA (ctDNA) from plasma and urine of patients with a metastatic prostate cancer.
The benefit of PARPi has been well established for ovarian (SOLO-1 study) and prostate cancer (PROFOUND study) with defects in the Homologous Recombination Repair (HRR) system due to BRCA1 or BRCA2 variants. Somatic variants in HRR genes are currently researched by Next Generation Sequencing (NGS). However, in metastatic prostate cancer, using formalin-fixed and paraffin-embedded (FFPE) samples, failure rate is around 30 % according to our retrospective datas, in agreement with the data of the PROFOUND study, highlighting a real pre-analytical matter when FFPE samples are used for NGS testing. Research of such alterations on circulating tumor DNA (ctDNA) extracted from plasma or urine could be a promising alternative test.
Study Type
OBSERVATIONAL
Enrollment
40
2 tubes Cell free DNA are taken during a blood sample already planned in the patient's care.
Urine sample is taken during the consultation carried out for the patient's care
University hospital
Tours, France
Circulating plasma tumour DNA
search for somatic variants of HRR genes (including BRCA1/BRCA2)
Time frame: Baseline
Circulating urine tumour DNA
search for somatic variants of HRR genes (including BRCA1/BRCA2)
Time frame: Baseline
FFPE tissue
search for somatic variants of HRR genes (including BRCA1/BRCA2)
Time frame: Baseline
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