Aim of the study was to determine if signaling pathways in placentas of mothers affected by overweight/obesity or by gestational diabetes are altered compared to placentas of a control group (normal weight, no gestational diabetes). Moreover, maternal blood and umbilical cord blood were analysed.
Study Type
OBSERVATIONAL
Enrollment
247
no intervention
University Hospital of Cologne
Cologne, North Rhine-Westphalia, Germany
Activity of Wnt signaling pathway in placental tissue
Analysis of components of the Wnt signaling pathway; like LRP6 receptor, beta-Catenin, GSK3beta, Wnt ligands (=proteins of interest) and an appropriate protein for normalization. Total protein lysates of placental tissue will be used. Analysis will be performed by western blotting and subsequent densitometric analysis. Outcome measure will be \[relative protein level of protein or interest/relative protein level of normalization protein\].
Time frame: through study completion, about 4 years in total
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.