This study is carried out in patients with IBD and healthy subjects requiring ileocolonoscopy as part of routine care (disease monitoring or polyp/colon cancer screening). It aims the generation and culturing of organoids from digestive biopsies recovered from healthy and/or pathological (inflammatory) ileal and/or colonic mucosa during an ileo-colonoscopy. These cultures will make it possible to validate the organoid production method used in the context of the research (primary objective). In a second phase (secondary objectives), the study will aim to setup a screening tool by irradiating the organoids (step one) and then evaluate in vitro the regenerative activity of treatments dedicated to improve inflammatory bowel diseases and acute radiation enteritis (step two).
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
16
endoscopy biopsies (6)
Groupe Hospitalier Ambroise Paré Hartmann
Neuilly-sur-Seine, Hauts-de-Seine, France
Validation of organoid-production method - quantity
Quantity of organoids created
Time frame: Day 1
Validation of organoid-production method - size
Size of organoids created
Time frame: Day 1
Validation of organoid-production method - cell growth
Cell-growth kinetics
Time frame: Day 1
Validation of organoid-production method - cell composition
Cell composition assessed by immunofluorescence
Time frame: Day 1
Validation of organoid-production method - cell composition
Cell composition compared to native-biopsy cell composition assessed by single-cell RNA sequencing
Time frame: Day 1
Validation of organoid-production method - cell apoptosis
Cell-apoptosis level assessed by immunofluorescence
Time frame: Day 1
Validation of organoid-production method - tight junctions
Number of tight junctions between cells assessed by immunofluorescence
Time frame: Day 1
Screening-tool setup - quantity
Quantity of organoids after irradiation
Time frame: Day 1
Screening-tool setup - size
Size of organoids after irradiation
Time frame: Day 1
Screening-tool setup - cell growth
Cell-growth kinetics after irradiation
Time frame: Day 1
Screening-tool setup - cell composition
Cell composition after irradiation assessed by immunofluorescence
Time frame: Day 1
Screening-tool setup - cell apoptosis
Cell-apoptosis level after irradiation assessed by immunofluorescence
Time frame: Day 1
Screening-tool setup - tight junctions
Number of tight junctions between cells after irradiation assessed by immunofluorescence
Time frame: Day 1
Screening-tool setup - inflammatory cytokines
Level of inflammatory-cytokines produced after irradiation assessed by single-cell RNA sequencing
Time frame: Day 1
In-vitro treatment evaluation - quantity
Quantity of organoids after irradiation and in presence of treatment
Time frame: Day 1
In-vitro treatment evaluation - size
Size of organoids after irradiation and in presence of treatment
Time frame: Day 1
In-vitro treatment evaluation - cell growth
Cell-growth kinetics after irradiation and in presence of treatment
Time frame: Day 1
In-vitro treatment evaluation - cell composition
Cell composition after irradiation and in presence of treatment assessed by immunofluorescence
Time frame: Day 1
In-vitro treatment evaluation - cell composition
Cell composition compared to native-biopsy cell composition assessed by single-cell RNA sequencing
Time frame: Day 1
In-vitro treatment evaluation - cell apoptosis
Cell-apoptosis level after irradiation and in presence of treatment assessed by immunofluorescence
Time frame: Day 1
In-vitro treatment evaluation - tight junction
Number of tight junctions between cells after irradiation and in presence of treatment assessed by immunofluorescence
Time frame: Day 1
In-vitro treatment evaluation - inflammatory cytokines
Level of inflammatory-cytokines produced after irradiation and in presence of treatment assessed by single-cell RNA sequencing
Time frame: Day 1
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