This study will compare efficacy of ovarian tissue freezing using slow-freeze versus vitrification methods.
The primary objective of this study is to determine the efficacy of ovarian tissue cryopreservation using slow-freeze and vitrification methods in enabling long-term preservation of ovarian function and fertility, in females undergoing treatment that may cause infertility. This study will provide study participants with their own tissue to cryopreserve and reserve for future use and: * Determine the efficacy of ovarian cryopreservation techniques. * Provide long-term follow up for patients who have undergone ovarian tissue cryopreservation for participants' own use. In addition to providing study participants with the future opportunity to use their tissue with the intent to reinstate ovarian function, this study will provide research tissue to the embryology research lab at the Family Fertility Center at Texas Children's Hospital that will be used to: * Optimize techniques for ovarian tissue cryopreservation of ovarian tissue for use in future retransplantation). * Investigate factors affecting successful maturation and quality of immature oocytes obtained from ovarian tissue including growth factors, hormones and other culture conditions.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
OTHER
Masking
NONE
Enrollment
24
Patients undergoing surgery for any indicated procedure for fertility-threatening treatment will have attempt at collecting ovarian tissue at time of surgery.
Texas Children's Hospital Pavilion For Women
Houston, Texas, United States
Effect of vitrification and slow freeze cryopreservation methods on follicular survival
Investigators will explore and compare the impact of vitrification and slow freeze cryopreservation methods by evaluating follicular survival using classic histological analysis of follicular morphology.
Time frame: 5+ years
Live birth rates in patients using cryopreserved ovarian tissue
Investigators will explore reproductive outcomes of women using cryopreserved tissue by measuring the live birth rate as defined by the number of initial live births divided by the total number of ovarian tissue implantations performed.
Time frame: 5+ years
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