Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in reproductive-aged women， which associated with increased risks to develop metabolic disorders, including cardiovascular diseases, diabetes mellitus, and cerebrovascular diseases. The precise pathogenesis of PCOS remains unknown but is thought to be multifactorial, comprising genetic and environmental factors .
To study the pathogenesis of diseases, the study of multi-omics approach has been applied in various diseases researching . Obesity and PCOS are both metabolic disorders, and both have an impact on metagenomics, metabolomics and transcriptome. Currently, multi-omics studies based on obese PCOS are still limited. Therefore, this study tried to use a multi-omics approach that integrates the gut microbiome, serum metabolomics, serum transcriptomics, and clinical indicators to distinguish obese PCOS from obese non-PCOS women. To investigate whether the specific composition of the gut microbiome is associated with PCOS in obese women. Serum metabolomics and transcriptomics were analyzed to explore potential metabolic pathways. Meanwhile, gut microbiome and serum metabolomics are used to predict the occurrence of PCOS. Then, a new metabolite that could predict PCOS were tested on female mice with high fat diet (HFD).
Study Type
OBSERVATIONAL
Enrollment
121
No intervention
Department of Endocrinology, Shanghai Tenth People's Hospital
Shanghai, Shanghai Municipality, China
Phenotype differences in the Serum non-targeted metabolomics by LC-MS.
Understand phenotypical differences from biological samples comparing women with and without PCOS. Venipuncture blood draws are to be completed and analyzed by a commercial laboratory to comprehensively analyze non-target metabolites.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
Phenotype differences in the gut microbiome by metagenomic sequencing.
Understand phenotypical differences from biological samples comparing women with and without PCOS. Stool samples are to be collected by women and analyzed with metagenomics sequencing.
Time frame: Samples to be collected once per participant within 7 days of enrollment
Phenotype differences in the transcriptome expression by mRNA-seq.
Understand phenotypical differences from biological samples comparing women with and without PCOS. The researcher will collect and analyze Venipuncture blood for RNA. Differences in the expression of genes between the two groups of subjects, analysis to select possible pathogenic factors leading to the pathogenesis of PCOS.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
Phenotype differences in the Serum targeted metabolomics by LC-MS.
Understand phenotypical differences from biological samples comparing women with and without PCOS. Venipuncture blood draws are to be completed and analyzed by a commercial laboratory to comprehensively analyze the target metabolite.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
BMI
Body mass index (BMI)=weight(kg)/height(m)\^2.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
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TT
total testosterone in nmol/L.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
LH
luteinizing hormone in IU/L.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
FSH
The follicle-stimulating hormone in IU/l.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
E2
Estradiol in pmol/L.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
PRL
Prolactin in uIU/ml.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
HC
Hip Circumference in centimeters.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
WC
Waist Circumference in centimeters.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
Waist/hip Ratio
WHR=(Waist Circumference in centimeters)/(Hip Circumference in centimeters).
Time frame: Samples to be collected once per participant within 7 days of enrollment.
Menstrual cycles
the total number of menstrual periods in the last year.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
FBG
fasting blood-glucose in mmol/L.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
PBG
postprandial blood-glucose in mmol/L.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
FINS
fasting serum insulin in mU/L.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
PINS
postprandial insulin in mU/L.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
ALT
alanine aminotransferase in U/L.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
AST
aspartate aminotransferase in U/L.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
UA
Uric acid in umol/L.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
CR
Creatinine in umol/L.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
Ferriman-Gallwey score
The minimum value of Ferriman-Gallwey score is 0 and the maximum value is 44. An Ferriman-Gallwey score greater than or equal to 6 is considered to be a clinical manifestation of androgen excess.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
HOMA-IR
Homeostatic model assessment insulin resistance index=FBG\*FINS/22.5.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
HbA1c (%)
glycosylated hemoglobin (%).
Time frame: Samples to be collected once per participant within 7 days of enrollment.
γ-GT
γ-glutamyl transferase in U/L.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
BUN
urea nitrogen in mmol/L.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
SOD
Superoxide dismutase in pg/ml.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
Interleukin 22, IL-22
Interleukin 22 in pg/ml. The factor reflects that the organism is in an inflammatory state. There is no maximum or minimum value for the factor and the higher scores mean a worse outcome.
Time frame: Samples to be collected once per participant within 7 days of enrollment.
Interleukin 6，IL-6
Interleukin 6 in pg/ml. The factor reflects that the organism is in an inflammatory state. There is no maximum or minimum value for the factor and the higher scores mean a worse outcome.
Time frame: Blood samples to be collected once per participant within 7 days of enrollment.
Interleukin 8， IL-8
Interleukin 8 in pg/ml. The factor reflects that the organism is in an inflammatory state. There is no maximum or minimum value for the factor and the higher scores mean a worse outcome.
Time frame: Blood samples to be collected once per participant within 7 days of enrollment.
Tumor Necrosis Factor， TNF
Tumor Necrosis Factor in pg/ml. The factor reflects that the organism is in an inflammatory state. There is no maximum or minimum value for the factor and the higher scores mean a worse outcome.
Time frame: Blood samples to be collected once per participant within 7 days of enrollment.
FT
free testosterone in nmol/L.
Time frame: Blood samples to be collected once per participant within 7 days of enrollment.
DHEAS
Dehydroepiandrosterone Sulfate in ug/dl.
Time frame: Blood samples to be collected once per participant within 7 days of enrollment.
SHBG
sex hormone-binding globulin in nmol/L.
Time frame: Blood samples to be collected once per participant within 7 days of enrollment.
AD
Androstenedione in ng/ml.
Time frame: Blood samples to be collected once per participant within 7 days of enrollment.
LDL-c
low-density lipoprotein cholesterol in mmol/L.
Time frame: Blood samples to be collected once per participant within 7 days of enrollment.
HDL-c
high-density lipoprotein cholesterol in mmol/L.
Time frame: Blood samples to be collected once per participant within 7 days of enrollment.
TC
Total Cholesterol in mmol/L.
Time frame: Blood samples to be collected once per participant within 7 days of enrollment.
TG
Triglyceride in mmol/L.
Time frame: Blood samples to be collected once per participant within 7 days of enrollment.
CRP
C reactive protein in pg/ml.
Time frame: Blood samples to be collected once per participant within 7 days of enrollment.