The aim of this project is to evaluate avenanthramides and avenacosides and their metabolites as specific biomarkers of oat product intake in humans. Biomarkers will be evaluated after i) a single dose through a single meal based on a solid and a liquid oat-based product (pharmacokinetic study) ii) week-long consumption at three different intake levels (dose-response). The study will be carried out as a two-way cross-over design with two different oat products, liquid or solid, where each product is provided 3 times per day during 5 days per product. A wash-out period of 8 days where participants consume their habitual diet is implemented. The first day of study meal intervention includes postprandial measurements during 8 hours.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
OTHER
Masking
SINGLE
Enrollment
23
Intervention products consist of two different oat products, one solid and one liquid. The content of oat in the products differs.
University of Gothenburg, Department of Food and Nutrition and Sport Science
Gothenburg, Sweden
Plasma concentrations of avenanthramides and avenacosides and their metabolites.
Difference in the plasma concentrations of diet specific biomarkers comparing before (baseline) and after intervention meal (average plasma concentration over a 24 h period).
Time frame: 24 hours
Plasma concentration-time profile over 24h (AUCs) of avenanthramides and avenacosides and their metabolites.
Differences in plasma AUCs between the intake levels for each biomarker candidate.
Time frame: 24 hours
Urine concentrations of avenanthramides and avenacosides and their metabolites
Difference in the plasma concentrations of diet specific biomarkers comparing before (baseline) and after intervention meal (average urine concentration over a 8 h period).
Time frame: 8 hours
Gut microbiome
Fecal samples will analyzed for composition of the gut microbiome, baseline compared with after 5 days of intervention products.
Time frame: 5 days
Plasma metabolites
Untargeted metabolomics will be performed using established methods for plasma. Analyzed exploratorily using untargeted metabolomics to find potential biomarker panels that reflect oats product intake. Baseline compared with after intervention products.
Time frame: 5 days
Urine metabolites
Untargeted metabolomics will be performed using established methods for urine. Analyzed exploratorily using untargeted metabolomics to find potential biomarker panels that reflect oats product intake.
Time frame: 24 hours
Blood concentrations of avenanthramides and avenacosides and their metabolites
Evaluate new simple sampling techniques using dried blood spots.
Time frame: 8 hours
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