N600X Low Saturation Accuracy Validation

Inclusion Criteria: 1\. Subject must have the ability to understand and provide written informed consent 2 Adult subjects 18 to 50 years of age. 3\. Subject must be willing and able to comply with study procedures and duration. 4\. Subject is a non-smoker or who has not smoked within 2 days prior to the study. 5\. Any gender of any race. 6\. Cleared same day Health Assessment form and health screening. 7\. Cleared physical exam by a licensed physician, physician assistant, or advanced practice nurse (medical exam includes 12-lead ECG, medical history and blood test to show no sickle cell disease present) 8\. Successful Perfusion Index Ulnar/Ulnar+Radial Ratio test showing adequate collateral blood flow. Exclusion Criteria: 1. Subject with sickle cell disease. 2. Subject is considered as being morbidly obese (defined as BMI \>39.5) 3. Compromised circulation, injury, or physical malformation of fingers, hands, ears or forehead/skull or other sensor sites which would limit the ability to test sites needed for the study. (Note: Certain malformations may still allow subjects to participate if the condition is noted and would not affect the sites utilized.) 4. Females of childbearing potential, who are pregnant, who are trying to get pregnant or who have a urine test positive for pregnancy on the day of the study 5. Subjects with COHb levels \>3% as assessed by CO-Oximetry during the procedure 6. tHb \< 10 g/dl as assessed by CO-Oximetry during the procedure 7. MetHb ≥ 2% as assessed by CO-Oximetry during the procedure 8. Subjects with known respiratory conditions such as: 1. uncontrolled / severe asthma, 2. flu or influenza type infection 3. pneumonia / bronchitis, 4. shortness of breath / respiratory distress, 5. unresolved respiratory or lung surgery, 6. emphysema, COPD, lung disease 9. Subjects with known heart or cardiovascular conditions such as: 1. Hypertension: systolic \>140mmHg, or Diastolic \>90mmHg on 3 consecutive readings. 2. have had cardiovascular surgery 3. Chest pain (angina) 4. heart rhythms other than a normal sinus rhythm or respiratory sinus arrhythmia 5. previous heart attack 6. blocked artery 7. unexplained shortness of breath 8. congestive heart failure (CHF) 9. history of stroke 10. transient ischemic attack 11. carotid artery disease 12. myocardial ischemia 13. myocardial infarction 14. cardiomyopathy Self-reported health conditions as identified in the Health Assessment Form 1. diabetes, 2. uncontrolled thyroid disease, 3. kidney disease / chronic renal impairment, 4. history of seizures (except childhood febrile seizures), 5. epilepsy, 6. history of unexplained syncope, 7. recent history of frequent migraine headaches, 8. recent symptomatic head injury, within the last 2 months 9. Subjects with known clotting disorders 10. history of bleeding disorders or personal history of prolonged bleeding from injury 11. history of blood clots 12. hemophilia 13. current use of blood thinner: prescription or daily use of aspirin 14. Subjects with Severe contact allergies to standard adhesives, latex or other materials found in pulse oximetry sensors, ECG electrodes, respiration monitor electrodes or other medical sensors 15. Subjects with prior or known severe allergies to lidocaine (or similar pharmacological agents, e.g. Novocain) or heparin 16. Arterial cannulation within the last 30 days prior to study date, (this may exclude only one radial artery site. Left or right) 17. History of clinically significant complications from previous arterial cannulation. 18. A radial artery with ten or more arterial cannulations right or left, excludes that site. 19. Unwillingness or inability to remove colored nail polish or colored artificial nails other than clear from test digits. 20. Other known health condition, should be considered upon disclosure in Health Assessment form