Frozen Embryo Transfer Pregnancy Outcomes Prediction by Microbiome and Transcriptome

Inclusion Criteria: 1. Infertile women receiving assisted reproductive technology treatment, preparing for frozen embryo transfer cycle; 2. Female age: 20-45 years old; 3. Single frozen-thawed blastocyst Transferred 4. Endometrial thickness ≥7mm on the day of luteal transformation 5. Written informed consent. Exclusion Criteria: 1. Women who have pathologies or malformations that affect the pregnancy outcome:genital malformations, intrauterine adhesions, uterine fibroids (\>4 cm in diameter or compressing endometrial line), adenomyosis, endometrial polyps; untreated tubal pleura Presence of autoimmune disease; 2. Untreated hyperprolactinemia, thyroid disease, adrenal disease 3. Antibiotic use within the past 7 days; 4. Current or recent (within 3 months) drug abuse, including alcohol and tobacco; 5. Refused or unable to comply with protocol requirements; 6. Participation in any experimental drug study within 60 days prior to screening.