Change in Skin Surface Temperature at the Neck When Using Acupuncture at Houxi Point in Healthy Volunteers

Inclusion Criteria: * Healthy males and females were individual age between enough 18 and 30 years old. * BMI: 18.5 - 23 kg/m2 * Mental alertness, good contact, cooperation with researchers. * Having no psychiatric stress problem during acupuncture day (confirmed by answering the DASS21 questionnaire with stress point less than 15 points). * Vital sign within normal limits: * Heart rates from from 60 to 99 beats/minute. * Systolic blood pressure between 90 and 139 mmHg. * Diastolic blood pressure between 60 and 89 mmHg. * Body temperature: 36.59 ± 0.43 0C. * Breathing rate: 16 ± 3 respirations/minute . * Having no disease or using drugs that change the body temperature. * Not currently participating in other intervention studies. * Volunteers who agree to participate and sign the Informed Consent Form, following a detailed explanation of clinical trials. * Having no acupuncture knowledge and experience yet. Exclusion Criteria: * Playing sport 2 hours before the study. * Taking stimulant (alcohol, beer, coffee and tobacco) within 24 hours before conducting the study. * Staying up late at night or having insomnia before the day of the study. * Women who were in menstruation period, pregnancy, or breastfeeding. * Having skin injuries, dermatitis-skin infections or wounds in the area to be investigated. * Curently having neck-pain or other diseases that could affect or interfere with outcomes, including common cold. * Currently taking sedatives or drugs that cause vasodilation, affect blood pressure and heart rate. * Applying chemical or pharmaceutical products to the site of the skin to be investigated before conducting the study. * Using physical therapy, heat therapy, cupping therapy, massage, acupuncture in the site of the skin to be investigated within 24 hours. * Anxiety, depression, stress before conducting the study with DASS 21 score \< 15.