The aim of the study is to investigate whether the food matrix has an influence on the prebiotic efficacy (bifidobacteria growth) of chicory-derived Orafti® inulin in healthy adult volunteers. The main question it aims to answer is: Does the food matrix influence the prebiotic effect of chicory-derived inulin in terms of stimulation of bifidobacteria in the gut microbiota Participants will receive one of three food items containing inulin or pure inulin dissolved in water daily for 10 days. The food items or pure inulin powder will deliver 10 g/d inulin split into two dosages of 5 g/d. They will provide stool samples before and at the end of the intake period. They will record any gastrointestinal sensations and bowel habits in a diary. Urinary samples will be collected at baseline and end of intervention for analysis of metabolites with Nuclear Magnetic resonance (NMR).
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
OTHER
Masking
NONE
Enrollment
96
Each intervention provided 10 g/d inulin split into two dosages of 5 g.
University of Reading
Reading, United Kingdom
Changes in level of Bifidobacterium spp.
Bifidobacterium spp. will be assessed by fluorescence in situ hybridisation - flow cytometry (FISH-FLOW) and 16S rRNA gene sequencing
Time frame: 10 days
Changes in level of total Bacteria
Total bacteria will be assessed by fluorescence in situ hybridisation - flow cytometry (FISH-FLOW)
Time frame: 10 days
Changes in gut bacterial composition
Bacterial numbers will be assessed by fluorescence in situ hybridisation - flow cytometry (FISH-FLOW) and 16S rRNA gene sequencing
Time frame: 10 days
Stool frequency
Numbers of stools will be recorded in daily diary
Time frame: 10 days
Stool consistency
Stool consistency will be recorded in daily diary according to Bristol Stool Form Scale
Time frame: 10 days
Bloating
Bloating will be recorded in daily diary according to a 4-point scale
Time frame: 10 days
Abdominal pressure
Abdominal pressure will be recorded in daily diary according to a 4-point scale
Time frame: 10 days
Abdominal pain
Abdominal pain will be recorded in daily diary according to a 4-point scale
Time frame: 10 days
Flatulence
Flatulence will be recorded in daily diary according to a 4-point scale
Time frame: 10 days
Changes in urinary metabolites
Urinary metabolites will be analysed by Nuclear magnetic resonance (NMR)
Time frame: 10 days
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