The purpose of this study will be to assess the effectiveness of bioimpedance device (BIA) as a safe, rapid, and non-invasive method of analyzing muscle damage induced by high intensity induced eccentric exercise as an alternative measurement tool against traditional assessment methods. A sample of 28 sedentary subjects will be recruited, all participants will perform an eccentric exercise protocol. Measurements will be done before performing the exercise protocol, immediately after finishing the protocol, 3h after the end of the exercise protocol, 24h after the end of the exercise protocol and 48h after the end of the exercise protocol. The variables analyzed in all subjects will be phase angle, creatinkinase, jumping strength, VAS scale, white blood count cell, quadriceps muscle thickness, Borg scale and anxiety and depression questionnaires.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
DIAGNOSTIC
Masking
NONE
Enrollment
28
Excentric exercise protocol will be performed for all subjets, consisted of 10 sets of 20 repetitions of squats, jumping from a height of 50 cm
Universidad Francisco de Vitoria
Pozuelo de Alarcón, Madrid, Spain
RECRUITINGPhase angle
The phase angle, which is measured via bioelectrical impedance analysis (BIA), InBody 770 ,is a clinically important bioimpedance parameter used for nutritional assessment and evaluating the risk of various diseases. The phase angle was calculated automatically by the BIA device from these two components according to the following formula: phase angle (°) = (reactance/resistance) × (180°/π).
Time frame: change from baseline to 72 hours
Pain: VAS
Visual Analogic Scale. Pain will be measured with a visual analog scale from 0 to 10. 0 points is no pain.
Time frame: change from baseline to 72 hours
Quadriceps thickness
Quadriceps muscle thickness will be measures in mm. by an ultrasound device
Time frame: change from baseline to 72 hours
Creatinkinase (CK concentration)
CK concentration will be measure in mg/dl with a portal analyzer (SpotchemTM EZ SP-4430), using test strips.
Time frame: change from baseline to 72 hours
cellular inflammatory markers
Cellular inflammatory markers will be measured with hematology analyzer (Sysmex KX-21N Automed) in 10\^9/L and % White blood count, lymphocytes, neutrophils, monocytes, eosinophils, basophils will be analyzed
Time frame: change from baseline to 72 hours
Leg power
Leg power will be assessed using vertical jump test, with Chronojump platform (CHRONOJUMP Boscosystem)
Time frame: change from baseline to 72 hours
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