In this A Single-center, Prospective, Self-controlled trial, subjects should avoid a high-Zinc diet for 2 weeks and then take Zinc supplements for 2 weeks.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
56
Each participant should receive a regular zinc diet for weeks 1-2 and take zinc supplements for weeks 3-4.
Southern Medical Unversity，the seventh affiliated hospital
Foshan, Guangdong, China
16S rDNA sequencing
16S rDNA sequencing technology was used to identify the structural and functional changes of intestinal microbiota before and after Zinc supplementation.
Time frame: 14days，28 days
metabolomic analysis of serum and feces
To determine the alteration of gut microbiota after Zn supplementary.
Time frame: 14days，28 days
The blood levels of Zn2+ in each study phase
The blood levels of Zn2+ would be detected on day 14 and day 28.
Time frame: 14days，28 days
Number of participantswith abnormal laboratory tests results
If there are abnormal situations during the experiment that may pose significant risks to the health or safety of the participants, the research team may need to terminate the experiment to ensure participant safety. Only
Time frame: 14days，28 days
The fecal Zn2+ concentration in each study phase
The fecal Zn2+ concentration would be detected on day 14 and day 28.
Time frame: 14days，28 days
Total bilirubin concentration after 14days oral Zn.
change from 14days in Total bilirubin and analyzed by the AU5800 fully automatic biochemical analyzer.
Time frame: 14days，28 days
Direct bilirubin concentration after 14days oral Zn.
change from 14days in Direct bilirubin and analyzed by the AU5800 fully automatic biochemical analyzer.
Time frame: 14days，28 days
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Indirect bilirubin concentration after 14days oral Zn.
change from 14days in indirect bilirubin and analyzed by the AU5800 fully automatic biochemical analyzer.
Time frame: 14days，28 days
uric acid concentration after 14days oral Zn.
change from 14days in uric acid and analyzed by the AU5800 fully automatic biochemical analyzer.
Time frame: 14days，28 days
total triglyceride concentration after 14days oral Zn.
change from 14days in total triglyceride and analyzed by the AU5800 fully automatic biochemical analyzer.
Time frame: 14days，28 days
total protein concentration after 14days oral Zn.
change from 14days in total protein and analyzed by the AU5800 fully automatic biochemical analyzer.
Time frame: 14days，28 days
aspartate transaminase concentration after 14days oral Zn.
change from 14days in aspartate transaminase and analyzed by the AU5800 fully automatic biochemical analyzer.
Time frame: 14days，28 days
alanine transaminase concentration after 14days oral Zn.
change from 14days in alanine transaminase and analyzed by the AU5800 fully automatic biochemical analyzer.
Time frame: 14days，28 days
albumin concentration after 14days oral Zn.
change from 14days in albumin and analyzed by the AU5800 fully automatic biochemical analyzer.
Time frame: 14days，28 days
total cholesterol concentration after 14days oral Zn.
change from 14days in total cholesterol and analyzed by the AU5800 fully automatic biochemical analyzer.
Time frame: 14days，28 days
Urea concentration after 14days oral Zn.
change from 14 days in urea and analyzed by the AU5800 fully automatic biochemical analyzer.
Time frame: 14days，28 days
HDL-C concentration after 14days oral Zn.
change from 14 days in HDL-C(high density lipoprotein cholesterol) and analyzed by the AU5800 fully automatic
Time frame: 14days，28 days
LDL-C concentration after 14days oral Zn.
change from 14 days in LDC-C(low density lipoprotein cholesterol) and analyzed by the AU5800 fully automatic
Time frame: 14days，28 days
Red blood cells analysis after 14days oral Zn.
change from 14days in Red blood cells and determined by a Sysmex-xn-2000 automatic blood cell analyser.
Time frame: 14days，28 days
Plateltes analysis after 14days oral Zn.
change from 14 days in Plateltes and determined by a Sysmex-xn-2000 automatic blood cell analyser.
Time frame: 14days，28 days
Neutrophil cells analysis after 14days oral Zn.
change from 14days in Neutrophil cells and determined by a Sysmex-xn-2000 automatic blood cell analyser.
Time frame: 14days，28 days
Eosinophil cells analysis after 14days oral Zn.
change from 14days in Eosinophil cells and determined by a Sysmex-xn-2000 automatic blood cell analyser.
Time frame: 14days，28 days
Basophil cells analysis after 14days oral Zn.
change from 14 days in Basophil cells and determined by a Sysmex-xn-2000 automatic blood cell analyser.
Time frame: 14days，28 days
Lymphocyte cells analysis after 14days oral Zn.
change from 14 days in Lymphocyte cells and determined by a Sysmex-xn-2000 automatic blood cell analyser.
Time frame: 14days，28 days
Monocyte cells analysis after 14days oral Zn.
change from 14 days in Monocyte cells and determined by a Sysmex-xn-2000 automatic blood cell analyser.
Time frame: 14days，28 days
White blood cells analysis after 14days oral Zn.
change from 14 days in white blood cells and determined by a Sysmex-xn-2000 automatic blood cell analyser.
Time frame: 14days，28 days