Alteration of Symbiosis Intestinal Microbiota on Patients With Anorexia Nervosa

Assistance Publique - Hôpitaux de Paris120 enrolled

Overview

The purpose of this study will be to study the association between the level of psychic symptomatic of anorexia nervosa (AN) (intensity of food restriction, symptoms of anxiety and depression) and alteration of host environment symbiosis and the mechanism (dysbiosis of intestinal microbiota, increase of intestinal permeability, immunity alteration and low-grade inflammation).

This is a monocentric study aims to characterise the intestinal microbiota of anorexia nervosa patients with malnutrition, in comparison with the control subject, by DNA sequencing and metagenomic method. The interaction of intetinal microbiota with the host will be studied through the mecanistic studies and various parameters of alteration of symbiosis (intestinal permeability, inflammation) and their association with psychic symptoms of anorexia nervosa. The objective of this approche is to have not only a descrition of genomic of material of sampling, but also an overview of its potential functioning.

Study Type

OBSERVATIONAL

Enrollment

120

Conditions

Anorexia Nervosa Dysbiosis Anxious Depression

Interventions

Stool and blood samplesBIOLOGICAL

Stool and blood samples

Eligibility

Sex: ALLMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: For patients: * Patients aged ≥18 years; * Anorexia according to DSM-5 and CIDI (Composite International Diagnostic Interview); * Body mass index (BMI) (P/T2) \< 15; * Hospitalization for nutrition rehabilitation; * Covered by a health insurance; * Informed consent form signed. For Healthy Volunteers: * Aged ≥18 years; * 18.5 \< BMI (P/T2) \< 25; * Covered by a health insurance; * Informed consent form signed. Exclusion Criteria: For patients: * Patients no-responding all criteria of DSM-5 or CIDI scores; * Taken of antibiotic treatment 2 months / or laxativ 3 weeks before hospitalization; * Somatic comorbidity should perturb intestinal microbiota (Crohn's disease, diabetes and all other chronic inflammatory diseases); * Patients under guardianship; * Patients covered by french AME scheme. For Healthy Volunteers: * Any disease should perturb intestinal microbiota; * Recent ponderal variation; * Taken of antibiotic treatment 2 months or laxativ 3 weeks before hospitalization; * Under guardianship; * Covered by french AME scheme.

Locations (1)

Service de Nutrition Clinique, Hôpital Paul Brousse (APHP)

Villejuif, France

RECRUITING

Outcomes

Primary Outcomes

Eating Disorder

By Eating Disorder Inventory (EDI-3)

Time frame: at baseline

Anxio-depressive symptomatologic assessements by HAD score

By HAD score

Time frame: at baseline

Anxio-depressive symptomatologic assessements by scale of Beck BDI 13

By scale of Beck BDI 13

Time frame: at baseline

Anxio-depressive symptomatologic assessements by LSAS Lieboweitz social anxiety scale

By LSAS Lieboweitz social anxiety scale

Time frame: at baseline

Anxio-depressive symptomatologic assessements by MOCI

By Maudsley Obsessions and compulsions inventory (MOCI).

Time frame: at baseline

Anxio-depressive symptomatologic assessements

By HAD score, scale of Beck BDI 13, LSAS Lieboweitz social anxiety scale, Maudsley Obsessions and compulsions inventory (MOCI).

Time frame: at baseline

Physical exercise

By Global physical activity questionnaire (GPAQ)

Time frame: at baseline

Biological parameter

By serum serotonin level

Time frame: at baseline

Secondary Outcomes

Diversity indice

Central Contacts

Mouna HANACHI GUIDOUM, MD

CONTACT

+ 33 6 70 39 42 37 mouna.hanachi@aphp.fr

Joël DORE

CONTACT

+ 33 1 34 65 27 09 joel.dore@jouy.inrae.fr

Data from ClinicalTrials.gov

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